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Microscopic and proteomic analysis of Zea mays roots (P30F53 variety) inoculated with Azospirillum brasilense strain FP2

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Abstract

Plant growth-promoting rhizobacteria (PGPR) are considered to have a beneficial effect on host plants. Azospirillum brasilense is a diazotroph associated with important agricultural crops such as maize and wheat, and has been used as model organism to investigate associative plant growth promotion. In this study, we have employed scanning electronic microscopic to observe the presence of bacterium on the root surface. Two-dimensional gel electrophoresis and mass spectrometry were used to analyze the changes in protein profile of maize roots in response to A. brasilense strain FP2 7 days after inoculation and we observed 46 differently expressed protein spots. We report the identification of three of these proteins.

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Abbreviations

PGPR:

plant growth-promoting rhizobacteria

DAI:

days after inoculation

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Authors and Affiliations

  1. Department of Food Science and Technology, Federal University of Santa Catarina, Rod. Admar Gonzaga, 1346, 88034-001, Florianópolis-SC, Brazil

    Alexandro Cézar Faleiro, Pedro Alexandre Valentim Neto & Ana Carolina Maisonnave Arisi

  2. Department of Botany, Federal University of Santa Catarina, Florianópolis-SC, Brazil

    Thaysi Ventura de Souza & Marisa Santos

Authors
  1. Alexandro Cézar Faleiro
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  2. Pedro Alexandre Valentim Neto
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  3. Thaysi Ventura de Souza
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  4. Marisa Santos
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  5. Ana Carolina Maisonnave Arisi
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Correspondence to Ana Carolina Maisonnave Arisi.

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Faleiro, A.C., Neto, P.A.V., de Souza, T.V. et al. Microscopic and proteomic analysis of Zea mays roots (P30F53 variety) inoculated with Azospirillum brasilense strain FP2. J. Crop Sci. Biotechnol. 18, 63–71 (2015). https://doi.org/10.1007/s12892-014-0061-x

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  • DOI: https://doi.org/10.1007/s12892-014-0061-x

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