Abstract
Vitis amurensis is a species of wild grape with high ecological, medicinal, and economic values. Here, the complete chloroplast genome of this plant was assembled from whole-genome high-throughput sequencing data. The circular double-stranded DNA molecule is 160,953 bp in size, including a pair of inverted repeats (26,354 bp each) separated by large (89,187 bp) and small (19,058 bp) single-copy regions. The chloroplast genome contains 133 genes, including 88 protein-coding genes (80 PCG species), 37 transfer RNA genes (29 tRNA species) and eight ribosomal RNA genes (four rRNA species), 20 of which are duplicated, including eight protein-coding, eight tRNA, and four rRNA genes. The base composition is asymmetric (30.93 % A, 19.07 % C, 18.33 % G, 31.67 % T) with an overall A + T content of 62.60 %. A phylogenetic analysis based on complete chloroplast genome sequences showed that V. amurensis is closely related to V. vinifera, V. aestivalis, and V. rotundifolia.
References
Blasi P, Blanc S, Wiedemannmerdinoglu S, Prado E, Ruhl E, Mestre P, Merdinoglu D (2011) Construction of a reference linkage map of Vitis amurensis and genetic mapping of Rpv8, a locus conferring resistance to grapevine downy mildew. Theor Appl Genet 123:43–53. doi:10.1007/s00122-011-1565-0
Cronquist A (1982) An integrated system of classification of flowering plants, vol 216. Columbia University Press, New York, pp 1217–1218. doi:10.1126/science.216.4551.1217
Ha DT, Chen QC, Hung TM, Youn UJ, Ngoc TM, Thuong PH, Kim HJ, Seong YH, Min BS, Bae KH (2009) Stilbenes and oligostilbenes from leaf and stem of Vitis amurensis and their cytotoxic activity. Arch Pharm Res 32:177–183. doi:10.1007/s12272-009-1132-2
Hahn C, Bachmann L, Chevreux B (2013) Reconstructing mitochondrial genomes directly from genomic next-generation sequencing reads-a baiting and iterative mapping approach. Nucleic Acids Res 41:e129. doi:10.1093/nar/gkt371
Ingrouill MJ, Fls MWC, Fls MFF, Bowman D, Bank MVD, Bruijn ADE (2002) Systematics of Vitaceae from the viewpoint of plastid rbcL DNA sequence data. Bot J Linn Soc 138:421–432. doi:10.1046/j.1095-8339.2002.00028.x
Jansen RK, Kaittanis C, Saski C, Lee SB, Tomkins J, Alverson AJ, Daniell H (2006) Phylogenetic analyses of Vitis (Vitaceae) based on complete chloroplast genome sequences: effects of taxon sampling and phylogenetic methods on resolving relationships among rosids. BMC Evol Biol 6:32. doi:10.1186/1471-2148-6-32
Lee HR, Bak MJ, Jeong WS, Kim YC, Chung SK (2009) Antioxidant properties of proanthocyanidin fraction isolated from wild grape (Vitis amurensis) seed. Korean J Food Sci Technol 52:539–544. doi:10.3839/jksabc.2009.091
Li D, Wan Y, He P (2008) Relatedness of resistance to anthracnose and to white rot in Chinese wild grapes. VITIS 47:213–215
Lohse M, Drechsel O, Kahlau S, Bock R (2013) OrganellarGenomeDRAW—a suite of tools for generating physical maps of plastid and mitochondrial genomes and visualizing expression data sets. Nucleic Acids Res 41:W575–W581. doi:10.1093/nar/gkt289
Ma YY, Zhang YL, Shao H, Lu J (2009) Differential physio-biochemical responses to cold stress of cold-tolerant and non-tolerant grapes (Vitis L.) from China. J Agron Crop Sci 196:212–219. doi:10.1111/j.1439-037X.2009.00405.x
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729. doi:10.1093/molbev/mst197
Wan Y, Schwaninger H, Li D, Simon C, Wang Y, Zhang C (2008) A review of taxonomic research on Chinese wild grape. VITIS 47:81–88
Weidner S, Karamać M, Amarowicz R, Szypulska E, Gołgowska A (2007) Changes in composition of phenolic compounds and antioxidant properties of Vitis amurensis seeds germinated under osmotic stress. Acta Physiol Plant 29:283–290. doi:10.1007/s11738-007-0035-4
Yim NH, Ha DT, Trung TN, Jin PK, Lee SM, Na MK, Jung H, Kim HS, Kim YH, Bae KW (2010) The antimicrobial activity of compounds from the leaf and stem of Vitis amurensis against two oral pathogens. Bioorg Med Chem Lett 20:1165–1168. doi:10.1016/j.bmcl.2009.12.020
Acknowledgments
This work was supported by the Agricultural Science and Technology Innovation Program (CAAS-ASTIP-2016-ZFRI) and Crop Resources Protection Program of China Ministry of Agriculture (NB2016-20130135-34).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Xie, H., Jiao, J., Fan, X. et al. The complete chloroplast genome sequence of Chinese wild grape Vitis amurensis (Vitaceae: Vitis L.). Conservation Genet Resour 9, 43–46 (2017). https://doi.org/10.1007/s12686-016-0615-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-016-0615-y