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Development of eight polymorphic microsatellite markers in the Black and Rufous sengi, Rhynchocyon petersi

  • Microsatellite Letters
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Abstract

The Black and Rufous sengi, Ryhnchocyon petersi, is endemic to a limited range in East Africa. We report the development of eight polymorphic microsatellites using next generation sequencing technology. Eighteen individuals from Zaraninge forest (Saadani National Park, Tanzania) were genotyped. The number of alleles per locus ranged from 2 to 6, while the observed and expected heterozygosities varied from 0.17 to 0.82 and from 0.25 to 0.81, respectively. No locus deviated from Hardy–Weinberg equilibrium. These microsatellite markers will be useful tools to study the effect of habitat fragmentation on the population genetic structure of R. petersi.

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References

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Acknowledgments

The authors wish to thank all persons involved in the realisation of this study. Special thank goes to the field assistants Omari Kibwana, Dege Hussein, Victor Morris and Samweli Shaba for their commitment during the whole period of data collections. Tanzania Wildlife Research Institute and Tanzania National Parks gave permission to conduct the study. The staffs of Saadani National Park are thanked for their support especially Mr Hassani Ngulume, Chief Park Warden and Miss Halima Penga, Warden for Ecology. Funding was obtained through the VLIR—Own Initiative Program. Galen Rathbun provided valuable comments on methodology on field collections of the study species. JGB is a postdoctoral fellow with FWO.

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Authors and Affiliations

  1. Pest Management Centre, Sokoine University of Agriculture, Box 3110, Morogoro, Tanzania

    Christopher A. Sabuni & Rhodes H. Makundi

  2. Evolutionary Ecology Group, University of Antwerp, Groenenborgerlaan 171, 2020, Antwerp, Belgium

    Natalie Van Houtte, Herwig Leirs & Joëlle Goüy de Bellocq

  3. Department of Wildlife Management, Sokoine University of Agriculture, Box 3073, Morogoro, Tanzania

    Samwel L. S. Maganga

  4. Research Facility Studenec, Institute of Vertebrate Biology, Academy of Sciences of the Czech Republic, Květná 8, Brno, Czech Republic

    Joëlle Goüy de Bellocq

Authors
  1. Christopher A. Sabuni
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  2. Natalie Van Houtte
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  3. Samwel L. S. Maganga
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  4. Rhodes H. Makundi
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  5. Herwig Leirs
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  6. Joëlle Goüy de Bellocq
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Corresponding author

Correspondence to Joëlle Goüy de Bellocq.

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Sabuni, C.A., Van Houtte, N., Maganga, S.L.S. et al. Development of eight polymorphic microsatellite markers in the Black and Rufous sengi, Rhynchocyon petersi . Conservation Genet Resour 7, 193–195 (2015). https://doi.org/10.1007/s12686-014-0330-5

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  • DOI: https://doi.org/10.1007/s12686-014-0330-5

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