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Development of 16 microsatellite loci for the endangered sandhill dunnart (Sminthopsis psammophila)

  • Microsatellite Letters
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Abstract

Sixteen polymorphic microsatellite loci were developed using a 454 sequencing approach, and three loci, developed for Sminthopsis douglasi, were optimized for the endangered sandhill dunnart, Sminthopsis psammophila. Nineteen loci (16 S. psammophila and three S. douglasi) were tested on 38 individual S. psammophila from two populations in South Australia. Three loci deviated from Hardy–Weinberg equilibrium, possibly due to null alleles. No evidence of linkage disequilibrium was detected. We recommend a final panel of 17 loci for future genetic analyses of S. psammophila, which will aid in conservation management of this poorly understood and nationally endangered marsupial.

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Acknowledgments

Funding was obtained from OneSteel Whyalla, Nature Foundation SA, Holswoth Wildlife Research Endowment, Australian and Pacific Science Foundation and Field Naturalists Society of South Australia. We thank the Department of Environment, Water and Natural Resources for access to Ironstone Hill Conservation Park, Matthew Ward (DEWNR) for collecting tissue samples from Yellabinna Regional Reserve and Kathy Saint for laboratory assistance.

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Correspondence to Amanda L. McLean.

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McLean, A.L., Cooper, S.J.B., Lancaster, M.L. et al. Development of 16 microsatellite loci for the endangered sandhill dunnart (Sminthopsis psammophila). Conservation Genet Resour 6, 323–326 (2014). https://doi.org/10.1007/s12686-013-0084-5

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  • DOI: https://doi.org/10.1007/s12686-013-0084-5

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