Skip to main content
SpringerLink
Log in

A set of 18 polymorphic microsatellite loci in the Algerian mouse, Mus spretus Lataste 1883 (Rodentia: Muridae)

  • Technical Note
  • Published:
Conservation Genetics Resources Aims and scope Submit manuscript

Abstract

A multiplex PCR assay was developed for the identification of polymorphic microsatellite loci (18) for the Algerian mouse, Mus spretus, using 454 GS-FLX Titanium pyrosequencing. The utility of these markers was evaluated in 184 individuals from for different Moroccan populations. Number of alleles per locus ranged from 10 to 39, with expected and observed heterozygosities ranging from 0.256 to 0.947 and from 0.259 to 0.926 respectively. None departures from Hardy–Weinberg equilibrium were observed at all loci. These markers will be valuable tools for population genetic studies in conservation management.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Da Silva Junior F, Monarca RI, Dias D, Ramalhinho M, Mathias ML, Mucillo-Baisch AL (2012) Physiological damage in Algerian mouse Mus spretus (Rodentia: Muridae) exposed to crude oil. J Biosci Biotechnol 1(2):125–133

    Google Scholar 

  • Jombart T (2008) adegenet: a R package for the multivariate analysis of genetic markers. Bioinformatics 24:1403–1405

    Article  CAS  PubMed  Google Scholar 

  • Khidas K, Khammes N, Khelloufi S, Lek S, Aulagnier S (2002) Abundance of the wood mouse Apodemus sylvaticus and the Algerian mouse Mus spretus (Rodentia, Muridae) in different habitats of Northern Algeria. Mammalian Biol 67:34–41

    Google Scholar 

  • Malausa T, Gilles A, Meglecz E, Blanquart H, Duthoy S, Costedoat C, Dubut V, Pech N, Castagnone-Sereno P, Delye C, Feau N, Frey P, Gauthier P, Guillemaud T, Hazard L, Le Corre V, Lung-Escarmant B, Male P-J, Ferreira S, Martin J-F (2011) High-throughput microsatellite isolation through 454 GS-FLX Titanium pyrosequencing of enriched DNA libraries. Mol Ecol Resour 11:638–644

    Article  CAS  PubMed  Google Scholar 

  • Rousset F (2008) Genepop’007: a complete re-implementation of the genepop software for Windows and Linux. Mol Ecol Resour 8:103–106

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

This study was supported by the ‘Service de Systématique Moléculaire’ of the ‘Muséum National d’Histoire Naturelle’ (CNRS—UMS 2700) and ANR 6ème extinction ANR-09-PEXT-004, MOHMIE, ‘MOdern Human installation in Morocco, Influence on the small terrestrial vertebrate biodiversity and Evolution’ (C. Denys).

Author information

Authors and Affiliations

  1. Département Systématique et Evolution, UMR CNRS 7205, Muséum National d’Histoire Naturelle, CP 51, 75231, Paris Cedex 05, France

    Aude Lalis

  2. Laboratoire Génome Populations Interactions, UMR CNRS 5000, Université Montpellier-II, 34095, Montpellier Cedex 05, France

    Annie Orth

Authors
  1. Aude Lalis
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Annie Orth
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Aude Lalis.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lalis, A., Orth, A. A set of 18 polymorphic microsatellite loci in the Algerian mouse, Mus spretus Lataste 1883 (Rodentia: Muridae). Conservation Genet Resour 6, 255–258 (2014). https://doi.org/10.1007/s12686-013-0080-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12686-013-0080-9

Keywords

Search

Navigation