Abstract
Eighteen novel microsatellite markers were developed in Rhododendron ferrugineum using pyrosequencing technology. They were tested on populations in the Alps, Jura and Pyrenees to study genetic diversity at the species range margins. Protection of populations at margins is important for conserving the genetic diversity of species. We found 2–7 alleles per locus. The expected and observed heterozygosity ranged from 0.07 to 0.79 and from 0.05 to 0.85, respectively. These loci were successfully amplified in 22 other Rhododendron species and four Ericaceae species. These markers will be useful for investigating the genetic structure of R. ferrugineum and related species at their distribution margins.
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Acknowledgments
We thank F. Forest and E. Kapinos (Royal Botanic Gardens, Kew) and G. Argent (RBG, Edimburgh) for providing Rhododendron species DNA. This study has been supported by a research program of the “Fondation pour la Recherche sur la Biodiversité” (COMBIO project No. AAP-IN-2009-013) and by a grant from the French National Institute for Agricultural Research (INRA), AIP BioRessources EcoMicro. This work has been done in the lab EDB, part of the “Laboratoire d’Excellence (LABEX) entitled TULIP (ANR-10-LABX-41).
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Charrier, O., Delmas, C.E.L., Pornon, A. et al. Development of 18 microsatellite markers in Rhododendron ferrugineum (Ericaceae) for investigating genetic structure at margins. Conservation Genet Resour 5, 473–477 (2013). https://doi.org/10.1007/s12686-012-9831-2
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DOI: https://doi.org/10.1007/s12686-012-9831-2