Abstract
Twenty-nine microsatellite loci were isolated and characterized from the genome of Esomus metallicus. Flanking polymerase chain reaction primers were designed and used to amplify these loci in 32 individuals. All loci were polymorphic; with allele numbers ranged from 2 to 27, observed heterozygosity from 0.031 to 0.969 and expected heterozygosity from 0.031 to 0.957. All conformed to Hardy–Weinberg equilibrium and no evidence of null alleles was observed. Pairwise comparisons between alleles did not detect any cases of linkage disequilibrium. The high level of polymorphisms observed in these microsatellite loci should enhance future investigation of the genetic differentiation and structure of populations of E. metallicus.
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Acknowledgments
This study was conducted in part as a cooperative survey of the Japan International Research Center for Agricultural Sciences (JIRCAS) and the Living Aquatic Resources Research Center, Lao PDR (LARRC). Our thanks go to Mses. Poutsamone Pommachan and Phonaphet Chantasone for their assistance in the field research, and to Mses. Kyoko Yamanoi and Ponthip Goto for their supports in chemical analyses. This study was supported in part by a Grant-in-Aid for Scientific Research (C-23580340, B-22405030 and B-22380133) from the Japan Society for the Promotion of Science.
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Koizumi, N., Morioka, S., Quinn, T.W. et al. Development and characterization of 29 polymorphic microsatellite loci for Esomus metallicus . Conservation Genet Resour 4, 1027–1030 (2012). https://doi.org/10.1007/s12686-012-9699-1
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DOI: https://doi.org/10.1007/s12686-012-9699-1