Abstract
Eleven polymorphic microsatellite loci were obtained from Hoplodactylus duvaucelii using high-throughput genomic sequencing technology. Loci isolated from H. duvaucelii showed extensive cross-amplification for H. maculatus, resulting in six polymorphic loci for H. duvaucelii and 10 for H. maculatus. Genetic diversity was assessed in 34 individuals of H. duvaucelii from Korapuki Island, and 20 individuals of H. maculatus from Kapiti Island.
References
Abdelkrim J, Robertson BC, Stanton JL, Gemmell N (2009) Fast, cost-effective development of species-specific microsatellite markers by genomic sequencing. Biotechniques 46:185–191
Chambers G, Boon W, Buckley T, Hitchmough R (2001) Using molecular methods to understand the Gondwanan affinities of the New Zealand biota: three case studies. Aust J Bot 49:377–387
Faircloth BC (2008) MSATCOMMANDER: detection of microsatellite repeat arrays and automated, locus-specific primer design. Mol Ecol Resour 8:92–94
Oosterhout C, Hutchinson W, Willds D, Shipley P (2004) MICROCHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538
Peakall R, Smouse PE (2006) GENALEX 6: genetic analysis in excel. Population genetic software teaching and research. Mol Ecol Notes 6:288–295
Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249
Rice WR (1989) Analysing tables of statistical tests. Evolution 43:223–225
Towns DR (1994) The role of ecological restoration in the conservation of Whitaker’s skink (Cyclodina whitakeri), a rare New Zealand lizard (Lacertilia: Scinicidae). J Zool 21:457–471
Towns DR (2002) Korapuki Island as a case study for restoration of insular ecosystems in New Zealand. J Biogeogr 29:593–607
Towns DR, Atkinson IAE (2004) Restoration plan for Korapuki Island (Mercury Islands), New Zealand, 2004–2024. Department of Conservation, Wellington
Towns DR, Daugherty CH, Cree A (2001) Raising the prospects for a forgotten fauna: a review of 10 years of conservation effort for New Zealand reptiles. Biol Conserv 99:3–16
Acknowledgments
Funding for this project was provided by Massey University. We thank Marleen Baling for field assistance, Diana Prada, Ana Ramon-Laca and Birgit Ziesemann for laboratory assistance. The protocols in this research have been endorsed by Massey University Animal ethics committee (MUAEC 08/56) and New Zealand Department of Conservation (No-23041-FAU, WE/313/RES).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Wong, C., Gleeson, D., Howitt, R. et al. Polymorphic microsatellite loci and interspecific cross-amplification in the New Zealand endemic gecko species Hoplodactylus duvaucelii and Hoplodactylus maculatus . Conservation Genet Resour 3, 331–333 (2011). https://doi.org/10.1007/s12686-010-9356-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-010-9356-5