Abstract
Eleven microsatellite loci for the freshwater mussel Lasmigona costata were isolated and characterized. Genetic screening of individuals from four rivers in southern Ontario confirmed that all loci were highly polymorphic. These loci should prove useful in a range of studies from fine- and coarse-scale population structure and gene flow to reproductive success of individuals.
References
Berg DJ, Haag WR, Guttman SI, Sickel JB (1995) Mantle biopsy: a technique for nondestructive tissue-sampling of freshwater mussels. J North Am Benthol Soc 14:577–581
Bogan AE, Roe KJ (2008) Freshwater bivalve (Unioniformes) diversity, systematics, and evolution: status and future directions. J North Am Benthol Soc 27:349–369
Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. Method Enzymol 395:202–222
Lydeard C, Cowie RH, Ponder WF, Bogan AE, Bouchet P, Clark SA, Cummings KW, Frest TJ, Gargominy O, Herbert DG, Hershler R, Perez KE, Roth B, Seddon M, Strong EE, Thompson FG (2004) The global decline of nonmarine mollusks. Bioscience 54:321–330
NatureServe (2009) NatureServe explorer: an online encyclopedia of life. www.natureserve.org/explorer. Accessed Aug 2009
Park SDE (2001) Trypanotolerance in West African cattle and the population genetic effects of selection. Ph.D. dissertation, University of Dublin
Raymond M, Rousset F (1995) GENEPOP (Version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249
Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments: a poor man’s approach to genotyping for research and high-throughput diagnostics. Nat Biotechnol 18:233–234
Williams JD, Warren MLJ, Cummings KW, Harris JL, Neves RJ (1993) Conservation status of freshwater mussels of the United States and Canada. Fisheries 18:6–22
Acknowledgments
This work was made possible through funding from the Endangered Species Recovery Fund of World Wildlife Fund Canada, Fisheries and Oceans Canada, and Ontario Ministry of Natural Resources. Friedrich Fischer and Ryan and Kirsty Hill provided valuable assistance with field sampling.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Galbraith, H.S., Wozney, K.M., Smith, C.M. et al. Isolation and characterization of microsatellite loci in the freshwater mussel Lasmigona costata (Bivalvia: Unionoida). Conservation Genet Resour 3, 9–11 (2011). https://doi.org/10.1007/s12686-010-9261-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-010-9261-y