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Isolation and characterization of 19 new microsatellites for European grayling, Thymallus thymallus (Linnaeus, 1758), and their cross-amplification in four other salmonid species

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Abstract

The European grayling (Thymallus thymallus) is a locally endangered and economically important freshwater salmonid fish that is distributed across a large part of Europe. Here we report 19 polymorphic microsatellites, doubling the number of available markers. Of those, 17 loci amplified in the closely related Arctic grayling (T. arcticus). Seven, six and ten amplified in the more distantly related salmonids Atlantic salmon (Salmo salar), Arctic charr (Salvelinus alpinus) and brown trout (Salmo trutta), respectively.

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Acknowledgments

We thank Hanna Heikelä, Niina Wahlroos and Mirkka Heinonen for excellent technical assistance, Nicola Barson for helpful discussions and Thrond Haugen and Jorma Piironen for supplying samples. CJ received support for a Marie Curie PhD fellowship awarded to the CEES. This study was financially supported by the Norwegian Research Council and the Academy of Finland.

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Correspondence to E. H. Leder.

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Junge, C., Primmer, C.R., Vøllestad, L.A. et al. Isolation and characterization of 19 new microsatellites for European grayling, Thymallus thymallus (Linnaeus, 1758), and their cross-amplification in four other salmonid species. Conservation Genet Resour 2 (Suppl 1), 219–223 (2010). https://doi.org/10.1007/s12686-009-9147-z

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  • DOI: https://doi.org/10.1007/s12686-009-9147-z

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