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Isolation and characterization of 16 microsatellite markers from a rare and endemic species, Scutellaria austrotaiwanensis (Lamiaceae)

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Abstract

Scutellaria austrotaiwanensis is a new species first described in 1997. It is a rare and endemic species in southern Taiwan. In this study, 16 polymorphic microsatellite loci were isolated from Scutellaria austrotaiwanensis using a magnetic bead enrichment method. Several genetic indices, including observed heterozygosity (Ho), expected heterozygosity (He), number of effective alleles (Ne), and polymorphism information content (PIC), were calculated. Deviation of heterozygosity was detected by the fixation index (Fis) and Hardy–Weinberg equilibrium was tested by possibilities (P HWE) using a chi-squared test. Allele numbers ranged from 4 to 8, with observed heterozygosity ranging from 0.611 to 1.000. Five microsatellite markers showed deficient heterozygosity by Fis > 0. No loci exhibited a departure from Hardy–Weinberg equilibrium, possibly due to a stable population. No loci pairs revealed significant linkage disequilibrium. The microsatellite markers reported here should be useful to evaluate the population structure of S. austrotaiwanensis for conservation efforts.

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Acknowledgments

We would like to thank Ms. Tzu-Hsiu Shen and Ms. Shih-Yin Chou for assistance with screening microsatellite loci. This study was supported by grants from the National Science Council of the ROC on Taiwan awarded to Yu-Chung Chiang.

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Authors and Affiliations

  1. Endemic Species Research Institute, Nantou, 552, Taiwan

    Tsai-Wen Hsu

  2. School of Nursing, Mei-Ho Institute of Technology, Pingtung, 912, Taiwan

    Chia-Chi Kuo

  3. Kaohsiung District Agricultural Improvement Station, Pingtung, 900, Taiwan

    Chi-Chu Tsai

  4. Department of Life Sciences, National Pingtung University of Science and Technology, Neipu, Pingtung, 912, Taiwan

    Yu-Chung Chiang

Authors
  1. Tsai-Wen Hsu
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  2. Chia-Chi Kuo
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  3. Chi-Chu Tsai
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  4. Yu-Chung Chiang
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Corresponding author

Correspondence to Yu-Chung Chiang.

Additional information

Tsai-Wen Hsu, Chia-Chi Kuo and Chi-Chu Tsai contributed equally to this work.

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Hsu, TW., Kuo, CC., Tsai, CC. et al. Isolation and characterization of 16 microsatellite markers from a rare and endemic species, Scutellaria austrotaiwanensis (Lamiaceae). Conservation Genet Resour 1, 85–88 (2009). https://doi.org/10.1007/s12686-009-9020-0

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  • DOI: https://doi.org/10.1007/s12686-009-9020-0

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