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Evaluation of the Effectiveness of Genetic Markers of Mycobacteria for Assessing the Disinfection Quality by Viability Real Time PCR

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Abstract

The research was aimed to evaluate the effectiveness of genetic markers when assessing the vitality of mycobacteria after room disinfection as a disinfection quality parameter. To achieve this goal, the mycobacterial genome was analyzed, and genetic markers identifying mycobacteria and marker mycobacterial products with the shortest lifetime after a mycobacterial cell dies were sought for. A room where animals were experimentally infected with mycobacteria was examined. Mycobacterium and the Mycobacterium tuberculosis complex DNA markers can be detected in one tube in CY5 and R6G channels. To differentiate Mycobacterium bovis BCG and the Mycobacterium tuberculosis complex, DNA deletion was detected using ROX- and R6G-labeled PCR probes. The comparison of genetic identification and mycobacterial vitality evaluation results with those of bacteriological tests completely confirm the effectiveness of genetic detection of mycobacterial viability. This approach to evaluate the disinfection quality makes it possible to avoid false-positive (using classic PCR) and false-negative (using bacteriologic culturing) results when controlling the tuberculosis causative agents.

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Acknowledgements

This study was supported by the Russian Government Program of Competitive Growth of Kazan Federal University.

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Authors and Affiliations

  1. Federal Center for Toxicological, Radiation and Biological Safety, Nauchniy Gorodok-2, Kazan, Tatarstan, Russian Federation, 420075

    Nail I. Khammadov & Eduard A. Shuralev

  2. Kazan Federal University, 18 Kremlyovskaya St., Kazan, Tatarstan, Russian Federation, 420008

    Natalya M. Aleksandrova, Alfiya V. Khammadova & Eduard A. Shuralev

  3. Russian Medical Academy of Continuous Professional Education (Kazan State Medical Academy Branch), 36 Butlerova St., Kazan, 420012, Russian Federation

    Eduard A. Shuralev

Authors
  1. Nail I. Khammadov
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  2. Natalya M. Aleksandrova
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  3. Alfiya V. Khammadova
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  4. Eduard A. Shuralev
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Correspondence to Nail I. Khammadov.

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Research Involving Humans and Animals Statement

No animals were used in this study.

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Khammadov, N.I., Aleksandrova, N.M., Khammadova, A.V. et al. Evaluation of the Effectiveness of Genetic Markers of Mycobacteria for Assessing the Disinfection Quality by Viability Real Time PCR. BioNanoSci. 9, 918–927 (2019). https://doi.org/10.1007/s12668-019-00654-8

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  • DOI: https://doi.org/10.1007/s12668-019-00654-8

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