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Spent Mushroom Substrates Valorization via Brand New Multirecycled Polyfunctional Biologics Producing on Them

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Abstract

Contemporary technologies although use various waste in polyfunctional biologics producing are high-cost and not waste-free. The new approaches in valorization waste based on biorecycling in lengthening the food chain and expanding the food network are eligible. The research goal was to develop new multiconversion biotechnology for the polyfunctional biologics’ production. Methods of semi-industrial liquid- and solid-phase sequential edible mushrooms and biologics producer strains cultivating were used as well as serial dilutions method for titer determining, quantity and quality of inoculums and preparative forms assessing, the content assessing methods for crude protein and basic polysaccharides, statistical processing. There have been developed the know-hows for the brand new 15 Multirecycled substrate-associated polyfunctional biologics (MSAPB) production. New substrates are developed for the producer strains growth via double spent mushroom substrates conversion. Their valorization already was observed in mushrooms double cultivation. Then 22 producer strains demonstrated high mushroom wastes’ multibioconversion efficiency as valorized bioinoculants. The MSAPB experimental samples shelf life was optimized in 18 months with preserving the producer strains viability and target activity. Such biologics put into the soil as bioinoculants and biopesticides for crop protection can restore and improve soil’s suppressiveness by optimizing the microbial complex in the agricultural plants’ rhizosphere.

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Acknowledgements

The author is grateful to the FSBSI ARI Testing Laboratory staff for their invaluable research assistance. The author very much appreciates the timely assistance of her personal sponsors and editors Valeria and Christopher Robert Hearsey, Oleg Darakov.

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  1. Federal State Budget Scientific Institution “All-Russian Research Institute of Plant Protection” (FSBSI VIZR), 3, Podbelskogo shosse, Pushkin, St. Petersburg, Russia, 196608

    Julia Anatolievna Titova

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Titova, J.A. Spent Mushroom Substrates Valorization via Brand New Multirecycled Polyfunctional Biologics Producing on Them. Waste Biomass Valor 13, 1089–1100 (2022). https://doi.org/10.1007/s12649-021-01552-5

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