Abstract
Polymorphisms in microRNAs (miRNAs) are associated with ischemic stroke occurrence and traditional risk factors for ischemic stroke such as atherosclerosis, hypertension, hyperlipidemia, and diabetes. However, few studies have examined recurrent ischemic stroke as an outcome. Thus, the aim of our study was to examine association of miRNA gene polymorphisms (namely, miR-126 rs4636297, miR-149 rs2292832, miR-124 rs531564, miR-499 rs3746444, miR-143 rs12655723, and miR-122 rs17669) with recurrence of ischemic stroke. miRNA gene polymorphisms were genotyped using the polymerase chain reaction–ligation detection reaction (PCR–LDR) method in 657 patients with ischemic stroke. Association of miRNA polymorphisms with prognosis outcomes was examined by the Kaplan–Meier method, log-rank test, and Cox proportional hazards models. miR-122 rs17669 was significantly associated with recurrence risk of ischemic stroke under the recessive model. Cox regression analysis showed that the CC genotype of rs17669 was associated with an increased risk of 1.9-fold for stroke recurrence (hazard ratio = 1.879; 95% confidence interval = 1.182–2.985; P = 0.008). Further, this effect was more evident among the non-drinker and male subgroups. We found no difference in risk of recurrent ischemic stroke among the other five miRNA polymorphisms. Furthermore, we identified a significant association between the miR-149 rs2292832 polymorphism and age at onset of first-ever stroke. Altogether, miR-122 rs17669 is a significant predictor for the risk of recurrent stroke, independent of traditional risk factors.
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Data Availability
The data used in our study are available from the authors upon reasonable request.
Abbreviations
- CT:
-
computed tomography
- DWI:
-
diffusion weighted imaging
- LAA:
-
large artery atherosclerosis
- SVD:
-
small vessel disease
- VCAM-1:
-
vascular cell adhesion molecule-1
- FOXO3:
-
forkhead box O3A,
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Acknowledgments
We are deeply grateful to all participants of this study.
Funding
This work was supported by the National Natural Science Foundation of China (81501006, 81400950). The design collection and analysis were supported by the funding.
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Designed the experiments: Ruixia Zhu and Xu Liu
Collected data: Qianwen Wang and Xu Liu
Wrote the paper: Ruixia Zhu
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All the patients signed informed consent for participation and the study was approved by Ethics Committees of the First Affiliated Hospital of China Medical University and conform to The Code of Ethics of the World Medical association.
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Liu, X., Wang, Q. & Zhu, R. Influence of miRNA Gene Polymorphism on Recurrence and Age at Onset of Ischemic Stroke in a Chinese Han Population. Neurotox Res 37, 781–787 (2020). https://doi.org/10.1007/s12640-019-00125-8
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DOI: https://doi.org/10.1007/s12640-019-00125-8