Abstract
During adolescence, the medial prefrontal cortex (mPFC) is still developing. We have previously shown that developmental cocaine exposure alters mPFC’s ability to cope with challenging events. In this manuscript, we exposed rats developmentally treated with cocaine to a novelty task and analyzed the molecular changes of mPFC. Rats were exposed to cocaine from post-natal day (PND) 28 to PND 42 and sacrificed at PND 43, immediately after the novel object recognition (NOR) test. Cocaine-treated rats spent more time exploring the novel object than saline-treated counterparts, suggesting an increased response to novelty. The messenger RNA (mRNA) and protein levels of the immediate early gene Arc/Arg3.1 were reduced in both infralimbic (IL) and prelimbic (PL) cortices highlighting a baseline reduction of mPFC neuronal activity as a consequence of developmental exposure to cocaine. Intriguingly, significant molecular changes were observed in the IL, but not PL, cortex in response to the combination of cocaine exposure and test such as a marked upregulation of both Arc/Arg3.1 mRNA and protein levels only in cocaine-treated rats. As for proteins, such increase was observed only in the post-synaptic density and not in the whole homogenate, suggesting psychostimulant-induced changes in trafficking of Arc/Arg3.1 or an increased local translation. Notably, the same profile of Arc/Arg3.1 was observed for post-synaptic density (PSD)-95 leading to the possibility that Arc/Arg3.1 and PSD-95 bridge together to promote aberrant synaptic connectivity in IL cortex following repeated exposure to cocaine during brain development.
Similar content being viewed by others
References
Bevins RA, Besheer J (2006) Object recognition in rats and mice: a one-trial non-matching-to-sample learning task to study ‘recognition memory’. Nat Protoc 1(3):1306–1311
Bolla KI, Eldreth DA, London ED, Kiehl KA et al (2003) Orbitofrontal cortex dysfunction in abstinent cocaine abusers performing a decision-making task. NeuroImage 19(3):1085–1094
Boulougouris V, Dalley JW, Robbins TW (2007) Effects of orbitofrontal, infralimbic and prelimbic cortical lesions on serial spatial reversal learning in the rat. Behav Brain Res 179(2):219–228
Bramham CR, Worley PF, Moore MJ, Guzowski JF (2008) The immediate early gene arc/arg3.1: regulation, mechanisms, and function. J Neurosci 28(46):11760–11767
Caballero A, Granberg R, Tseng KY (2016) Mechanisms contributing to prefrontal cortex maturation during adolescence. Neurosci Biobehav Rev 70:4–12
Caffino L, Calabrese F, Giannotti G, Barbon A et al (2015a) Stress rapidly dysregulates the glutamatergic synapse in the prefrontal cortex of cocaine-withdrawn adolescent rats. Addict Biol 20(1):158–169
Caffino L, Di Chio M, Giannotti G, Venniro M et al (2016) The modulation of BDNF expression and signalling dissects the antidepressant from the reinforcing properties of ketamine: effects of single infusion vs. chronic self-administration in rats. Pharmacol Res 104:22–30
Caffino L, Giannotti G, Malpighi C, Racagni G et al (2014) Long-term abstinence from developmental cocaine exposure alters Arc/Arg3.1 modulation in the rat medial prefrontal cortex. Neurotox Res 26(3):299–306
Caffino L, Giannotti G, Malpighi C, Racagni G et al (2015b) Short-term withdrawal from developmental exposure to cocaine activates the glucocorticoid receptor and alters spine dynamics. Eur Neuropsychopharmacol 25(10):1832–1841
Caffino L, Racagni G, Fumagalli F (2011) Stress and cocaine interact to modulate Arc/Arg3.1 expression in rat brain. Psychopharmacology 218(1):241–248
Casey BJ, Getz S, Galvan A (2008) The adolescent brain. Dev Rev 28(1):62–77
Chapman RH, Stern JM (1978) Maternal stress and pituitary-adrenal manipulations during pregnancy in rats: effects on morphology and sexual behavior of male offspring. J Comp Physiol Psychol 92(6):1074–1083
Chudasama Y, Passetti F, Rhodes SE, Lopian D et al (2003) Dissociable aspects of performance on the 5-choice serial reaction time task following lesions of the dorsal anterior cingulate, infralimbic and orbitofrontal cortex in the rat: differential effects on selectivity, impulsivity and compulsivity. Behav Brain Res 146(1–2):105–119
Colzato LS, Huizinga M, Hommel B (2009) Recreational cocaine polydrug use impairs cognitive flexibility but not working memory. Psychopharmacology 207(2):225–234
Dalley JW, Laane K, Pena Y, Theobald DE et al (2005) Attentional and motivational deficits in rats withdrawn from intravenous self-administration of cocaine or heroin. Psychopharmacology 182(4):579–587
DePoy LM, Gourley SL (2015) Synaptic cytoskeletal plasticity in the prefrontal cortex following psychostimulant exposure. Traffic 16(9):919–940
Ernst M, Romeo RD, Andersen SL (2009) Neurobiology of the development of motivated behaviors in adolescence: a window into a neural systems model. Pharmacol Biochem Behav 93(3):199–211
Farris S, Lewandowski G, Cox CD, Steward O (2014) Selective localization of arc mRNA in dendrites involves activity- and translation-dependent mRNA degradation. J Neurosci 34(13):4481–4493
Fumagalli F, Bedogni F, Frasca A, Di Pasquale L et al (2006) Corticostriatal up-regulation of activity-regulated cytoskeletal-associated protein expression after repeated exposure to cocaine. Mol Pharmacol 70(5):1726–1734
Fumagalli F, Caffino L, Racagni G, Riva MA (2009) Repeated stress prevents cocaine-induced activation of BDNF signaling in rat prefrontal cortex. Eur Neuropsychopharmacol 19(6):402–408
Fumagalli F, Moro F, Caffino L, Orru A et al (2013) Region-specific effects on BDNF expression after contingent or non-contingent cocaine i.v. self-administration in rats. Int J Neuropsychopharmacol 16(4):913–918
Garavan H, Hester R (2007) The role of cognitive control in cocaine dependence. Neuropsychol Rev 17(3):337–345
Giannotti G, Caffino L, Calabrese F, Racagni G et al (2013) Dynamic modulation of basic fibroblast growth factor (FGF-2) expression in the rat brain following repeated exposure to cocaine during adolescence. Psychopharmacology 225(3):553–560
Giannotti G, Caffino L, Calabrese F, Racagni G et al (2014) Prolonged abstinence from developmental cocaine exposure dysregulates BDNF and its signaling network in the medial prefrontal cortex of adult rats. Int J Neuropsychopharmacol 17(4):625–634
Giannotti G, Caffino L, Malpighi C, Melfi S et al (2015) A single exposure to cocaine during development elicits regionally-selective changes in basal basic fibroblast growth factor (FGF-2) gene expression and alters the trophic response to a second injection. Psychopharmacology 232(4):713–719
Hearing MC, Miller SW, See RE, McGinty JF (2008) Relapse to cocaine seeking increases activity-regulated gene expression differentially in the prefrontal cortex of abstinent rats. Psychopharmacology 198(1):77–91
Hearing MC, Schwendt M, McGinty JF (2011) Suppression of activity-regulated cytoskeleton-associated gene expression in the dorsal striatum attenuates extinction of cocaine-seeking. Int J Neuropsychopharmacol 14(6):784–795
Hester R, Garavan H (2009) Neural mechanisms underlying drug-related cue distraction in active cocaine users. Pharmacol Biochem Behav 93(3):270–277
Hosking JG, Cocker PJ, Winstanley CA (2016) Prefrontal cortical inactivations decrease willingness to expend cognitive effort on a rodent cost/benefit decision-making task. Cereb Cortex 26(4):1529–1538
Madsen HB, Zbukvic IC, Luikinga SJ, Lawrence AJ, et al. (2016). Extinction of conditioned cues attenuates incubation of cocaine craving in adolescent and adult rats. Neurobiol Learn Mem.
Moorman DE, James MH, McGlinchey EM, Aston-Jones G (2015) Differential roles of medial prefrontal subregions in the regulation of drug seeking. Brain Res 1628(Pt A):130–146
Palkovits M (1985) Microdissection of individual brain nuclei and areas. In: Boulton AA, Baker GB (eds) General neurochemical techniques. Humana Press, Totowa, NJ, pp. 1–17
Sierra-Mercado D, Padilla-Coreano N, Quirk GJ (2011) Dissociable roles of prelimbic and infralimbic cortices, ventral hippocampus, and basolateral amygdala in the expression and extinction of conditioned fear. Neuropsychopharmacology 36(2):529–538
Spear LP (2000) The adolescent brain and age-related behavioral manifestations. Neurosci Biobehav Rev 24(4):417–463
Spronk DB, van Wel JH, Ramaekers JG, Verkes RJ (2013) Characterizing the cognitive effects of cocaine: a comprehensive review. Neurosci Biobehav Rev 37(8):1838–1859
Torregrossa MM, Corlett PR, Taylor JR (2011) Aberrant learning and memory in addiction. Neurobiol Learn Mem 96(4):609–623
van den Bos R, Koot S, de Visser L (2014) A rodent version of the Iowa Gambling Task: 7 years of progress. Front Psychol 5:203
Verdejo-Garcia A, Bechara A, Recknor EC, Perez-Garcia M (2006) Executive dysfunction in substance dependent individuals during drug use and abstinence: an examination of the behavioral, cognitive and emotional correlates of addiction. J Int Neuropsychol Soc 12(3):405–415
Vonmoos M, Hulka LM, Preller KH, Jenni D et al (2013) Cognitive dysfunctions in recreational and dependent cocaine users: role of attention-deficit hyperactivity disorder, craving and early age at onset. Br J Psychiatry 203(1):35–43
Zavala AR, Osredkar T, Joyce JN, Neisewander JL (2008) Upregulation of Arc mRNA expression in the prefrontal cortex following cue-induced reinstatement of extinguished cocaine-seeking behavior. Synapse 62(6):421–431
Acknowledgements
We would like to thank the Zardi Gori Foundation for funding this project through a grant to FF.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Procedures involving animals and their care were conducted in conformity with institutional guidelines that are in compliance with national (D.L. n. 116, G.U., supplement 40, 18 Febbraio, 1992, Circolare No. 8, G.U., 14 Luglio, 1994) and international laws and policies (EEC Council Directive 2010/63/UE; Guide for the Care and Use of Laboratory Animals, National Academies Press, 8th Edition, 2011)
Conflict of Interest
The authors declare that they have no conflict of interest
Rights and permissions
About this article
Cite this article
Caffino, L., Giannotti, G., Mottarlini, F. et al. Developmental Exposure to Cocaine Dynamically Dysregulates Cortical Arc/Arg3.1 Modulation in Response to a Challenge. Neurotox Res 31, 289–297 (2017). https://doi.org/10.1007/s12640-016-9683-8
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12640-016-9683-8