Abstract
A myxozoan parasite belonging to the genus Thelohanellus Kudo, 1933 (Myxosporea, Bivalvulida) was isolated from the gill lamellae of Labeo bata (Hamilton, 1822) inhabiting Ranjit Sagar Wetland in Northern parts of Punjab, India. Histopathology of the infected gills indicated lamellar distortion due to the presence of plasmodia. The intensity of infection was recorded to be moderate as indicated by gill plasmodial index (GPI = 2). The plasmodia were microscopic, ovoid, creamish white, 2.0–4.0 mm in diameter containing 300–400 number of myxospores. The myxospores were broadly pyriform in shape, measuring 8.27 × 4.41 µm, having a prominent pore at the anterior end. The polar capsule was pyriform in shape, measuring 4.15 × 4.10 µm, containing a polar filament coiled perpendicular to the longitudinal axis of myxospore body making 6–7 turns. At molecular level, the analysis of 18S rDNA sequence of T. theinensis n. sp. demonstrated maximum homogeneity of 99 % with T. filli (KR340464) as only 2 gaps were found due to the difference of the last 20 base pairs at the 3′ end of T. theinensis n. sp. The current study emphasizes the need for caution when dealing with members of species complex.
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Abraham TJ, Banerjee S, Patra A, Sarkar A, Adikesavalu H, Dash G (2015) Molecular phylogeny of Myxobolus orissae (Myxosporea: Myxobolidae) infecting the gill lamellae of mrigal carp Cirrhinus mrigala (Actinopterygii: Cyprinidae). Mol Biol Res Comm 4(1):15–24
Andree KB, Székely C, Molnár K, Gresoviac SJ, Hedrick PP (1999) Relationships among members of the genus Myxobolus (Myxozoa: Bivalvidae) based on small subunit ribosomal DNA sequences. J Parasitol 85:68–74
Atkinson SD, Bartošová- Sojková P, Whipps CM, Bartholomew JL (2015) Approaches for characterising Myxozoan Species. In: Okamura B et al (eds) Myxozoan evolution, ecology and development. Springer International Publishing, Switzerland, pp 111–123
Attri R (2015) Taxonomic evaluation of myxozoan parasites infecting fresh water fishes in Punjab. Ph. D thesis Department of Zoology and Environmental Sciences, Punjabi University, Patiala (Punjab)
Banerjee S, Patra A, Adikesavalu H, Mondal A, Abraham TJ (2015) The phylogenetic position of Myxobolus carnaticus (Myxozoa, Myxosporea, Bivalvulida) infecting gill lamellae of Cirrhinus mrigala (Hamilton, 1822) based on 18S rRNA sequence analysis. Mol Biol Res Comm 4(3):125–132
Barta JR, Martin DS, Liberator PA, Dashkevicz M, Anderson JW, Feighner SD, Elbrecht A, Perkins-Barrow A, Jenkins MC, Danforth HD, Ruff MD, Profous-Juchelka H (1997) Phylogenetic relationships among eight Eimeria species infecting domestic fowl inferred using complete small subunit ribosomal DNA sequences. J Parasitol 83:262–271
Bartošová P, Fiala I (2011) Molecular evidence for the existence of cryptic species assemblages of several myxosporeans (Myxozoa). Parasitol Res 108(3):573–583
Basu S, Haldar DP (1999) Thelohanellus bifurcata n. sp. a new species of the genus Thelohanellus from hybrid carps and checklist of the species of the genus described from Indian fishes. Proc Zool Soc Calcutta 52(1):115–124
Basu S, Haldar DP (2003) Observations on two new thelohanelloid species (Myxozoa: Bivalvulida) from Indian major carps of West Bengal, India. J Parasit Appl Anim Biol 12(1&2):15–24
Basu S, Modak BK, Haldar DP (2015) Biological diversity of Thelohanellus Kudo, 1933 (Myxozoa: Myxosporea: Bivalvulida) parasitizing freshwater fishes of India. J Environ Sociobiol 1:26–27
Carriero MM, Adriano EA, Silva MRM, Ceccarelli PS, Maia AAM (2013) Molecular phylogeny of the Myxobolus and Henneguya Genera with several New South American Species. PLoS ONE 8(9):e73713. doi:10.1371/journal.pone.0073713
Chakravarty M, Basu MS (1948) Observations on some myxosporidians parasitic in fishes, with an account of nuclear cycles in one of them. Proc Zool Soc Bengal 1:23–33
Das MK, Halder DP, Mondal SK (1989) Macroecological studies on protozoan disease of carps in tropicalintensive fish culture ponds in India. Environ Ecology 7(1):1–9
Fiala I (2006) The phylogeny of Myxosporea (Myxozoa) based on small subunit ribosomal RNA gene analysis. Int J Parasitol 36:1521–1534
Kaur H, Attri R (2015) Prevalence and tissue specificity of Myxobolus saugati Kaur and Singh, 2011 (Myxozoa; Myxosporea; Bivalvulida) causing dermal myxoboliosis in wild and cultured Indian major carps in Punjab. Species 15:14–18
Kaur H, Gupta A (2015) Genetic relatedness provides support for a species complex of myxosporeans infecting the Indian major carp, Labeo rohita. Anim Biol 65:337–347
Kaur H, Katoch A (2014) Gill disease caused by Thelohanellus bifurcata Basu and Haldar, 1999 a pathogenic myxozoan parasite in cultured Indian carp, Labeo rohita (Hamilton, 1822) in Punjab, India. J Anim Health Prod 2:19–24
Kaur H, Singh R (2008) Observations on one new species of genus Myxobolus–M. naini and redescription of M. magauddi recorded from freshwater fishes of Kanjali Wetland of Punjab, India. In: Proceedings of 20th National Congress Parasitology, NEHU, Shillong, India, pp 75–79
Kaur H, Dar SA, Katoch A (2014a) Thelohanellus dykovi sp. nov. (Myxozoa: Bivalvulidae), a pathogenic gill parasite in cultured Indian major carp, Labeo rohita (Hamilton 1822) in Punjab (India). Species 10:24–30
Kaur H, Katoch A, Gupta M (2014b) Thelohanellus filli sp. n., a pathogenic myxosporean infecting gills of cultured carp, Labeo rohita (Hamilton 1822) in Punjab, India. Species 10:31–38
Kimura M (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120
Lalitha Kumari PS (1969) Studies on parasitic protozoa (Myxosporidia) of fresh water fishes of Andhra Pradesh, India. Riv Parasitol 30:153–226
Mohan CV, Shankar KM (1995) Pathogenic myxsporean infection in early fry of Indian major carp, Catla catla. Curr Sci 68(5):548–551
Molnár K (2002) Site preference of fish myxosporean in the gill. Dis Aquat Org 78:147–153
Mondal A, Banerjee S, Patra A, Adikesavalu H, Ramudu RK (2014) Molecular and morphometric characterization of Thélohanellus caudatus (Myxosporea: Myxobolidae) infecting the caudal fin of Labeo rohita (Hamilton). Parasitol 8(2):41–52
Pagarkar AU, Das M (1993) Two new species of myxozoa, Thelohanellus caudatus n. sp. and Myxobolus serrata n. sp. from cultural carps. J Inland Fish Soc India 25(1):30–35
Qadri SS (1962) A new Myxosporidian Thelohanellus boggoti n. sp. from an Indian fresh water fish Labeo boggot. Arch Protistenkd 106:218–222
Rajesh SC, Banerjee S, Patra A, Dash G, Abraham TH (2014) Molecular characterization of Myxobolus cuttacki (Myxozoa Myxosporea, Bivalvulida) infecting gill lamellae of minorcarp Labeo bata (Ham.). Mol Biol Res Comm 3(4):231–239
Ronquist F, Huelsenbeck JP (2003) Bayesian phylogenetic inference under mixed models. Biogeosciences 19:1572–1574
Sarkar NK (1985) Some coelozoic Myxosporida (Myxozoa: Myxosporea) from a freshwater water teleost fish of River Padma. Acta Protozool 24(1):47–53
Singh R, Kaur H (2012a) Biodiversity of myxozoan parasites infecting freshwater fishes of three main wetlands of Punjab, India. Protistol 7:79–89
Singh R, Kaur H (2012b) Thelohanellus (Myxozoa: Myxosporea: Bivalvulida) infections in major carp fish from Punjab wetlands (India). Protistol 7:178–188
Singh R, Kaur H (2012c) Myxosporean species of the genus Thelohanellus Kudo, 1933 (Myxozoa: Myxosprea: Bivalvulida) from freshwater fishes of Punjab wetlands, India. Protistol 7:208–217
Singh R, Kaur H (2012d) Two new Thelohanellus Kudo, 1933 (Myxozoa: Myxosprea: Bivalvulida) infecting fins of major carps in wetlands of Punjab. Trends Parasitol Res 1:34–43
Singh R, Kaur H (2014) Two new and two already known species of genus Thelohanellus Kudo, 1933 (Myxozoa: Myxosporea: Bivalvulida) infecting Indian major carp fishes in Punjab wetlands (India). J Parasitic Dis 38(1):49–60
Singh R, Kaur H (2015) Two new and one already known species of the genus Thelohanellus Kudo, 1933 (Myxozoa: Myxosporea: Bivalvulida) parasitizing fresh water fishes in wetlands of Punjab, India. Biologia 70:85–93
Szekely C, Molnar K, Cech G (2015) Description of Myxobolus balatonicus n. sp. (Myxozoa: Myxobolidae) from the common carp Cyprinus carpio L. in Lake Balton. Sys Parasitol 91(1):71–79
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2013) MEGA6: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance and maximum parsimony methods. Mol Biol Evol 28:2731–2739
Xi BW, Zhang JY, Xie J, Pan LK, Xu P, Ge XP (2013) Three actinosporean types (Myxozoa) from the oligochaete Branchirua sowerbyi in China. Parasitol Res 112:1575–1582
Yokoyama H, Grabner D, Shirakashi S (2012) Transmission biology of the myxozoa, health and environment in aquaculture. In: Carvalho E (ed) In Tech. http://www.intechopen.com/books/health-and-environin-aquaculture/transmission-biology-of-the-myxozoa
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The authors acknowledge financial support by University Grants Commission (UGC), Govt. of India under CAS grant to Department of Zoology, Panjab University, Chandigarh.
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Gupta, A., Kaur, H. Morphological, histopathological and molecular characterization of Thelohanellus theinensis n. sp. (Cnidaria: Myxosporea) infecting an Indian major carp, Labeo bata in a cold water wetland in Punjab (India). J Parasit Dis 41, 629–638 (2017). https://doi.org/10.1007/s12639-016-0856-4
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DOI: https://doi.org/10.1007/s12639-016-0856-4