Abstract
Three locally isolated strains of entomopathogenic nematodes (EPNs), viz. Heterorhabditis indica, Steinernema thermophilum and Steinernema glaseri, from Meghalaya, India were characterized in terms of storage temperature and survival and infectivity of their infective juveniles (IJs). The survival and infectivity of nematode IJs was studied at, 5 ± 2 and 25 ± 2 °C, for a period of 120 days, using deionized water as storage medium. The viability of nematode IJs was checked by mobility criterion at different storage periods, while the infectivity of nematode IJs was ascertained on the basis of establishment of IJs, using Galleria mellonella larva mortality tests in petridishes. The results of this study revealed that storage temperature markedly affects the survival as well as the establishment of nematode IJs of the three EPN species. At 5 °C, comparatively higher rate of IJ’s survival (i.e. 74–86 %) was observed for 15 days of storage, but the same reduced drastically to 28–32 % after 30 days of storage for H. indica and S. thermophilum. On the other hand, at 25 °C, the survival of nematode IJs was observed till 120 days for all the three studied EPNs. In case of S. thermophilum and S. glaseri, higher rate of IJs survival (>75 %) was observed respectively at 15 and 30 days of observation. The study also showed that the establishment of IJs of the three EPN species declines with increase in storage periods, at both the test temperatures. In general, the nematodes stored at 25 °C showed comparatively better establishment than those stored at 5 °C. Among the three EPN studied, the establishment of S. glaseri was comparatively better than the rest of the species at both the temperatures and for different storage durations. In conclusion, our study adds further valuable information about the effect of storage temperature on survival and infectivity of three indigenous EPN species of Meghalaya, India which appears to be promising biocontrol agents of local insect pests.
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References
Atkinson HJ (1980) Respiration in nematodes. In: Zuckerman BM (ed) Nematodes as biological models, vol 2. Academic Press, London, pp 101–142
Bedding RA (1981) Low cost in vitro mass production of Neoaplectana and Heterorhabditis species (Nematoda) for field control of insect pests. Nematologica 27:109–114
Bedding RA (1990) Logistics and strategies for introducing entomopathogenic nematode technology in developing countries. In: Gaugler R, Kaya HK (eds) Entomopathogenic nematodes in biological control. CRC Press, Boca Raton, pp 233–248
Cagnolo S, Campos V (2008) Effect of storage temperature on survival and infectivity of Steinernema rarum (OLI strain) (Rhabditida: Steinernematidae). J Invertebr Pathol 98:114–115
Fan X, Hominick WM (1991) Effects of low storage temperature on survival and infectivity of two Steinernema species (Nematoda: Steinernematidae). Rev Nématol 14:407–412
Ganguly S, Gavas R (2004a) Effect of soil moisture on the infectivity of entomopathogenic nematode, Steinernema thermophilum Ganguly & Singh. Int J Nematol 14:78–80
Ganguly S, Gavas R (2004b) Host range of entomopathogenic nematode, Steinernema thermophilum Ganguly and Singh (Steinernematidae: Rhabditida). Int J Nematol 14:221–228
Ganguly S, Singh LK (2001) Optimum thermal requirements for infectivity and development of an indigenous entomopathogenic nematode, Steinernema thermophilum Ganguly & Singh 2000. Indian J Nematol 31:148–152
Georgis R, Koppenhöfer AM, Lacey LA, Bélair G, Duncan LW, Grewal PS, Samish M, Tan L, Torr P, van Tol RWHM (2006) Successes and failures in the use of parasitic nematodes for pest control. Biol Control 38:103–123
Grewal PS (2000) Anhydrobiotic potential and long-term storage of entomopathogenic nematodes (Rhabditida: Steinernematidae). Int J Parasitol 30:995–1000
Grewal PS, Selvan S, Gaugler R (1994) Thermal adaptation of entomopathogenic nematodes: niche breadth for infection, establishment, and reproduction. J Therm Biol 19:245–253
Griffin CT (1993) Temperature responses of entomopathogenic nematodes for the success of biological control programs. In: Bedding RA, Akhurst RJ, Kaya HK (eds) Nematodes and the biological control of insect pests. CSIRO Publications, East Melbourne, pp 101–111
Hussaini SS (2003) Progress of research work on entomopathogenic nematodes in India. In: Hussaini SS, Rabindra RJ, Nagesh M (eds) Current status of research on entomopathogenic nematodes in India. Project directorate of biological control, Bangalore, pp 27–68
Katti G, Prasad JS, Padmakumari AP, Sankar M (2006) Effect of storage periods on survival and infectivity of indigenous entomopathogenic nematodes of insect pests of rice. Nematol Medit 34:37–41
Kaya HK (1990) Soil ecology. In: Gaugler R, Kaya HK (eds) Entomopathogenic nematodes in biological control. CRC Press, Boca Raton, pp 93–116
Kaya HK, Aguillera MM, Alumai A, Choo HY, de la Torre M, Fodor A, Ehlers RU (2006) Status of entomopathogenic nematodes and their symbiotic bacteria from selected countries of regions of the world. Biol Control 38:134–155
Koppenhöfer AM, Kaya HK (1999) Ecological characterization of Steinernema rarum. J Invertebr Pathol 73:120–128
Koppenhöfer AM, Kaya HK, Taormino SP (1995) Infectivity of entomopathogenic nematodes (Rhabditida: Steinernematidae) at different soil depths and moistures. J Invertebr Pathol 65:193–199
Lalramliana (2007) Faunal composition and distribution of entomopathogenic nematodes and their bioefficacy against major insect pests in Ri-bhoi district of Meghalaya. PhD Thesis, North-Eastern Hill University, Shillong, India
Lalramliana, Yadav AK (2009) Effect of relative humidity on the emergence and reproduction of three entomopathogenic nematode species (Rhabditida: Steinernematidae and Heterorhabditidae) from Meghalaya, India. Sci Vis 9:92–96
Nayak P, Rao YRVJ, Yadava CP, Rao YS (1977) Occurrence of a new entomophilic nematode on rice stem borer, Sesamia inferens. Oryza 14:51–54
O’Leary SA, Stack CS, Chubb MA, Burnell AM (1998) The effect of day of emergence from the insect cadaver on the behavior and environmental tolerances of infective juveniles of the entomopathogenic nematode Heterorhabditis megidis (strain UK211). J Parasitol 84:665–672
Poinar GO Jr (1979) Nematodes for biological control of insects. CRC Press, Boca Raton
Rahman PF, Sharma SB, Wightman JA (2000) A review of insect parasitic nematodes research in India: 1927-1997. Int J Pest Manag 46:19–28
Rao YR, PrakasaRao PS, Verma A, Israel P (1971) Tests with an insect parasitic nematode DD-136 (Nematoda: Steinemematidae) against rice stem borer, Tryporyza incertulas walk. Indian J Entomol 33:215–217
Singh SP (1977) Isolation of entomophilic nematode from potato cutworms. Curr Sci 46:454–455
Strauch O, Niemann I, Neumann A, Schmidt AJ, Peters A, Ehlers RU (2000) Storage and formulation of the entomopathogenic nematodes Heterorhabditis indica and H. bacteriophora. Biol control 45:483–500
Woodring JL, Kaya HK (1988) Steinernematid and heterorhabditid nematodes: a handbook of techniques southern cooperative series bulletin 331. Arkansas Agricultural Experiment Station, Fayatteville
Yadav AK, Lalramliana (2012a) Evaluation of the efficacy of three indigenous strains of entomopathogenic nematodes from Meghalaya, India against mustard sawfly, Athalia lugens proxima Klug (Hymenoptera: Tenthredinidae). J Parasit Dis 36:175–180
Yadav AK, Lalramliana (2012b) Efficacy of indigenous entomopathogenic nematodes from Meghalaya, India against the larvae of taro leaf beetle, Aplosonyx chalybaeus (Hope). J Parasit Dis 36:149–154
Yadav AK, Lalramliana (2012c) Soil moisture effects on the activity of three entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) isolated from Meghalaya, India. J Parasit Dis 36:94–98
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Authors thank the Head, Department of Zoology, NEHU, Shillong for providing the necessary research facilities. Lalramliana was recipient of UGC-CSIR, NET Research fellowship.
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Lalramliana, Yadav, A.K. Effects of storage temperature on survival and infectivity of three indigenous entomopathogenic nematodes strains (Steinernematidae and Heterorhabditidae) from Meghalaya, India. J Parasit Dis 40, 1150–1154 (2016). https://doi.org/10.1007/s12639-014-0639-8
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DOI: https://doi.org/10.1007/s12639-014-0639-8