Abstract
This study provides first results of transcriptome data for Cucumis sativus under sodium silicate (Si) supplementation, in order to identify differentially expressed genes (DEGs). In vitro-generated clonal C. sativus line B10 material, derived from leaf microexplants, was established and the plants were cultivated in Murashige and Skoog medium, non-supplemented and Si-supplemented with Na2(SiO2)xxH2O, respectively. The mRNA enrichment of pooled leaf and stem material from both the control and sodium Si-supplemented plants was performed, followed by RNA-Seq. Analysis of the cucumber transcriptome of the control and the Si-treated plants allowed for the determination of 18,957 and 18,882 transcripts referring to 19,896 genes. In total, 1,136 DEGs were determined and 522 (46 %) were assigned to biological processes, in most instances related to primary metabolism (photosynthesis, transport, biosynthesis), thereby supporting previous reports about the impact of Si on plant development, while some transcripts belong to secondary metabolism enabling subsequent analyses including stresses. Some transcripts were slightly up- or down-regulated and might be related to NaCl traces, due to the form of Si used in this instance. These transcriptome data provide the first insights into intermediate Si-accumulating cucumber tissue culture under sodium Sitreatment, with prior analyses on abiotic and biotic stresses.
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References
Sposito G (2008) The chemistry of soils. Oxford University Press
Epstein E (1999) Annu Rev Plant Biol 50(1):641–664
Epstein E (1994) Proc Natl Acad Sci USA 91(1):11–17
Mitani N, Ma J F (2005) J Exp Bot 56(414):1255–1261
Miyake Y, Takahashi E (1983) Soil Sci Plant Nutr 29(1):71–83
Liang Y, Si J, Römheld V (2005) New Phytol 167(3):797–804
Nikolic M, Nikolic N, Liang Y, Kirkby E A, Römheld V (2007) Plant Physiol 143(1):495–503
Ma J F, Yamaji N, Mitani N, Tamai K, Konishi S, Fujiwara T, Katsuhara M, Yano M (2007) Nature 448(7150):209–212
Chiba Y, Mitani N, Yamaji N, Ma J F (2009) Plant J 57(5):810–818
Mitani N, Yamaji N, Ma J F (2009) Plant Cell Physiol 50(1):5–12
Mitani-Ueno N, Yamaji N, Ma J F (2011) Plant Signal Behav 6(7):991
Deshmukh R K, Vivancos J, Guérin V, Sonah H, Labbé C, Belzile F, Bélanger R R (2013) Plant Mol Biol 83(4–5):303–315
Samuels A, Glass A, Ehret D, Menzies J (1991) Plant Cell Environ 14(5):485–492
Jana S, Jeong B R (2013) Trends Hortic Res 83(4–5):303–315
Sivanesan I, Park SW (2014) Front Plant Sci 5
Adatia M, Besford R (1986) Ann Bot 58(3):343–351
Burza W, Malepszy S (1995) Plant Breeding 114(4):341–345
Wóycicki R, Witkowicz J, Gawronski P, Dabrowska J, Lomsadze A, Pawelkowicz M, Siedlecka E, Yagi K, Plader W, Seroczynska A et al (2011) PLoS One 6(7):e22,728
Murashige T, Skoog F (1962) Physiol Plant 15(3):473–497
Alverson A J, Rice D W, Dickinson S, Barry K, Palmer J D (2011) Plant Cell 23(7):2499–2513
Pląder W, Yukawa Y, Sugiura M, Malepszy S (2007) Cell Mol Biol Lett 12(4):584–594
Koressaar T, Remm M (2007) Bioinformatics 23(10):1289– 1291
Kreps J A, Wu Y, Chang H S, Zhu T, Wang X, Harper J F (2002) Plant Physiol 130(4):2129–2141
Tomita Y, Mizuno T, Díez J, Naito S, Ahlquist P, Ishikawa M (2003) J Virol 77(5):2990–2997
Kolomiets M V, Chen H, Gladon R J, Braun E, Hannapel D J (2000) Plant Physiol 124(3):1121–1130
Ma J, Yamaji N (2008) Cell Mol Life Sci 65(19):3049–3057
Lee Y, Choi D, Kende H (2001) Curr Opin Plant Biol 4(6):527–532
Máthé C, Mosolygó Á, Surányi G, Beke A, Demeter Z, Tóth V R, Beyer D, Mészáros I, Márta M et al (2012) Aquat Bot 97(1):57–63
Fauteux F, Rémus-Borel W, Menzies J G, Bélanger R R (2005) FEMS Microbiol Ecol 249(1):1–6
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Holz, S., Kube, M., Bartoszewski, G. et al. Initial Studies on Cucumber Transcriptome Analysis under Silicon Treatment. Silicon 11, 2365–2369 (2019). https://doi.org/10.1007/s12633-015-9335-2
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DOI: https://doi.org/10.1007/s12633-015-9335-2