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Antibiotic Followed by a Potential Probiotic Increases Brown Adipose Tissue, Reduces Biometric Measurements, and Changes Intestinal Microbiota Phyla in Obesity

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Abstract

The development of adjuvant therapies for obesity treatment is justified by the high prevalence of this disease worldwide, and the relationship between obesity and intestinal microbiota is a promising target for obesity treatment. Therefore, this study aimed at investigating the adjuvant treatment of obesity through the use of potential probiotics and antibiotics, either separately or sequentially. In the first phase of the experiment, animals had diet-induced obesity with consumption of a high saturated fat diet and a fructose solution. After this period, there was a reduction in caloric supply, that is the conventional treatment of obesity, and the animals were divided into 5 experimental groups: control group (G1), obese group (G2), potential probiotic group (G3), antibiotic group (G4), and antibiotic followed by potential probiotic group (G5). The adjuvant treatments lasted 4 weeks and were administered daily, via gavage: Animals in G1 and G2 received distilled water, the G3 obtained Lactobacillus gasseri LG-G12, and the G4 received ceftriaxone. The G5 received ceftriaxone for 2 weeks, followed by the offer of Lactobacillus gasseri LG-G12 for another 2 weeks. Parameters related to obesity, such as biometric measurements, food consumption, biochemical tests, histological assessments, short-chain fatty acids concentration, and composition of the intestinal microbiota, were analyzed. The treatment with caloric restriction and sequential supply of antibiotics and potential probiotics was able to reduce biometric measures, increase brown adipose tissue, and alter the intestinal microbiota phyla, standing out as a promising treatment for obesity.

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Acknowledgements

The authors thank the Laboratório de Nutrição Experimental (DNS/UFV), Laboratório de Microbiologia de Anaeróbios (DMB/UFV) e Laboratório de Análises Clínicas (DNS/UFV), for making equipment and structure available for carrying out the analysis of this work.

Funding

The authors received financial support from the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and Fundação de Amparo à Pesquisa de Minas Gerais (FAPEMIG).

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  1. Department of Nutrition and Health, Universidade Federal de Viçosa, Avenue PH Rolfs S/N, Viçosa, Minas Gerais, 36570-900, Brazil

    Mariana de Moura e Dias, Sandra Aparecida dos Reis Louzano, Lisiane Lopes da Conceição, Rayssa da Conceição Fernandes, Solange Silveira Pereira & Maria do Carmo Gouveia Peluzio

  2. Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, Avenue PH Rolfs S/N, Viçosa, Minas Gerais, 36570-900, Brazil

    Tiago Antônio de Oliveira Mendes

  3. Department of General Biology, Universidade Federal de Viçosa, Avenue PH Rolfs S/N, Viçosa, Minas Gerais, 36570-900, Brazil

    Leandro Licursi de Oliveira

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MMD participated in all production stages of this article (handling the animals, execution of analysis, analysis of the results and writing of the article); SARL and RCF assisted in the handling of animals, in the execution of analyzes, and in writing of this article; LLC, TAOM, SSP, LLO, and MCGP guided in the choice of the analyzes performed, participated in the analysis of the results, and assisted in the writing of this article. All authors reviewed the manuscript.

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Correspondence to Mariana de Moura e Dias.

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The study was approved by the Ethics Committee on Animals Use of Universidade Federal de Viçosa, under protocol 33/2018. The principles recommended by the Nacional Council for the Control of Animal Experimentation were followed [41].

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de Moura e Dias, M., dos Reis Louzano, S.A., da Conceição, L.L. et al. Antibiotic Followed by a Potential Probiotic Increases Brown Adipose Tissue, Reduces Biometric Measurements, and Changes Intestinal Microbiota Phyla in Obesity. Probiotics & Antimicro. Prot. 13, 1621–1631 (2021). https://doi.org/10.1007/s12602-021-09760-0

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