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Lactobacillus rhamnosus JYLR-005 Prevents Thiram-Induced Tibial Dyschondroplasia by Enhancing Bone-Related Growth Performance in Chickens

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Abstract

Tibial dyschondroplasia (TD) is a leg disorder caused by the abnormal development of the tibia in fast-growing poultry. Lactobacillus rhamnosus (L. rhamnosus) strains have been reported to have effects on increasing bone growth and improving osteoporosis in animals. However, whether L. rhamnosus JYLR-005 can improve bone growth in TD chickens remains unclear. In this study, we noted that L. rhamnosus JYLR-005 could not reduce the suppression of the production performance of TD broilers (p > 0.05) but had a slight protective effect on the broiler survival rate (χ2 = 5.571, p = 0.062). However, for thiram-induced TD broiler chickens, L. rhamnosus JYLR-005 could promote tibia growth by increasing tibia-related parameters, including the tibia weight (day 11, p = 0.040), tibia length (day 15, p = 0.013), and tibia mean diameter (day 15, p = 0.035). Moreover, L. rhamnosus JYLR-005 supplementation improved the normal growth and development of the tibial growth plate by maintaining the morphological structure of the chondrocytes and restored the balance of calcium and phosphorus. Taken together, these findings provide a proof of principle that L. rhamnosus JYLR-005 may represent a therapeutic strategy to treat leg disease in chickens.

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Abbreviations

TD:

Tibial dyschondroplasia

L. rhamnosus :

Lactobacillus rhamnosus

GM:

Gut microbiota

ADFI:

Daily feed intake

ADG:

Average daily weight gain

Ca:

Calcium

P:

Phosphorus

Mg:

Magnesium

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Funding

This work was financially supported by the Outstanding Talents of Henan Agricultural University (No. 30500421).

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Author notes

  1. Fang Liu, Anan Kong and Pengfei Fu contributed equally to this work.

Authors and Affiliations

  1. College of Animal Science and Veterinary Medicine, Henan Agricultural University, 95# Wenhua Road, Jinshui District, Zhengzhou, 450002, Henan, People’s Republic of China

    Fang Liu, Anan Kong, Pengfei Fu, Qin-qin Cao, Kun-sheng Tao, Di-yi Liu, Xue-bing Wang, Zong-xi Tong & Shu-cheng Huang

  2. Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, People’s Republic of China

    Mujeeb Ur Rehman

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  1. Fang Liu
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Contributions

X.W., Z.T., M.U.R., and S.H. conceived and designed the experiments; A.K., Q.C., K.T., and D.L. performed the experiments; F.L., A.K., P. F., M.U.R., and S.H. analyzed the data; F.L., A.K., P. F., and S.H. wrote the paper.

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Correspondence to Shu-cheng Huang.

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Liu, F., Kong, A., Fu, P. et al. Lactobacillus rhamnosus JYLR-005 Prevents Thiram-Induced Tibial Dyschondroplasia by Enhancing Bone-Related Growth Performance in Chickens. Probiotics & Antimicro. Prot. 13, 19–31 (2021). https://doi.org/10.1007/s12602-020-09670-7

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