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Skimmed Milk-Based Encapsulation for Enhanced Stability and Viability of Lactobacillus gastricus BTM 7 Under Simulated Gastrointestinal Conditions

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Abstract

The present study investigated skimmed milk and alginate-based encapsulation for protection of a probiotic strain, Lactobacillus gastricus BTM7 during storage and exposure to simulated gastrointestinal conditions. The investigations have revealed that coating with skimmed milk and alginate in a ratio of 1:1 resulted in highest encapsulation efficiency of 94% (p < 0.05) with approximately 1 log reduction in viable cell count and 90% release of encapsulated cells in 90 min. This formulation resulted in 5-fold higher survival of bacteria during storage at refrigeration for 21 days (p < 0.05). The encapsulation of L. gastricus BTM7 provided better protection at the pH of gastric juice or pancreatic conditions with 4- and 9-fold increase in survivability after 2 h of incubation. The principal component analysis (PCA) revealed the potential of skimmed milk supplementation to alginate (1:1) to enhance survival of probiotic strain under refrigerated storage, a process that can be safely incorporated into dairy products.

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Acknowledgements

The authors wish to thank Jaypee University of information Technology for providing essential facilities required for this research work.

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Authors and Affiliations

  1. Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Solan, 173234, India

    Manisha Singh, Deepak Sharma, Rajni Chauhan & Gunjan Goel

  2. Department of Microbiology, School of Interdisciplinary and Applied Life Sciences, Central University of Haryana, Mahendergarh, Haryana, 123031, India

    Gunjan Goel

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  1. Manisha Singh
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  2. Deepak Sharma
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Correspondence to Gunjan Goel.

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Singh, M., Sharma, D., Chauhan, R. et al. Skimmed Milk-Based Encapsulation for Enhanced Stability and Viability of Lactobacillus gastricus BTM 7 Under Simulated Gastrointestinal Conditions. Probiotics & Antimicro. Prot. 11, 850–856 (2019). https://doi.org/10.1007/s12602-018-9472-1

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