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Effects of Probiotics on Survival, Growth and Digestive Enzymes Activities in Freshwater Prawn Macrobrachium rosenbergii (De Man 1879)

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Abstract

A study was conducted to examine the effects of three probiotics, Lactobacillus sporogenes, Bacillus subtilis and Saccharomyces cerevisiae on the survival, growth and digestive enzymes activities of the freshwater prawn Macrobrachium rosenbergii post larvae (PL). The probiotics, L. sporogenes (4 %), B. subtilis (3 %) and S. cerevisiae (4 %) were taken and mixed with basal diet. Diet without probiotics served as control. These probiotics diets were fed to M. rosenbergii PL for a period of 60 days. After the feeding trail, the growth parameters such as survival, weight gain, specific growth rate and protein efficiency rate were found to be significantly (P < 0.05) higher in 4 % S. cerevisiae incorporated diet fed PL when compared with control. In the case of feed conversion rate just the reverse was seen (P < 0.05) at this concentration. This indicates its superior quality among different concentrations of probiotics tested. Activities of digestive enzymes, such as protease, amylase and lipase were significantly (P < 0.05) higher at this concentration (4 % S. cerevisiae). Some of essential and non-essential amino acids also significantly elevated in probiotics supplemented diet fed prawns. This study indicated that probiotics, S. cerevisiae incorporated diets were beneficial for M. rosenbergii in terms of increasing growth, enzyme and amino acid production.

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References

  • Abe, H., and S. Ohmama. 1987. Effect of starvation, and sea-water acclimation on the concentration and free l-histidine and related dipeptides in the muscle of eel, rainbow trout and Japanese dace. Comparative Biochemistry and Physiology 88B: 507–511.

  • Ali, A. 2000. Probiotics in fish farming. Evaluation of a bacterial mixture. Ph.D. Thesis, University of Agricultural Sciences, Umea, Sweden.

  • Anderson, T., and S. De Silva. 2003. Nutrition. In Aquaculture: Farming of aquatic animals and plants, ed. J.S. Lucas and P.C. Southgate, 146–171. Victoria, Australia: Blackwell Publishing.

  • APHA. 2005. Standard methods for the examination of water and wastewater, 19th ed. New York: American Public Health Association.

    Google Scholar 

  • Babsky, E.B., B.I. Khodorov, G.I. Kositsky, and A.A. Zubkov. 1989. In Human physiology, ed. E.B. Babsky, Moscow: Mir Publishers.

  • Balcazar, J.L., I. De-Blas, I. Ruis-Zarzuela, D. Cunningham, D. Vendrell, and J.L. Muzquiz. 2006. The role of probiotics in aquaculture. Veterinary Microbiology 114: 173–186.

    Article  PubMed  Google Scholar 

  • Balcazar, J.L., T. Rojas-Luna, and T. Cunningham. 2007. Effect of the addition of four potential probiotic strains on the survival of pacific white shrimp (Litopenaeus vannamei) following immersion challenge with Vibrio parahaemolyticus. Journal of Invertebrate Pathology 96: 147–150.

    Article  PubMed  Google Scholar 

  • Barker, G. 1998. Novel alternatives to old chemotherapeutants-disease management section. Fish Farm 21: 16–20.

    Google Scholar 

  • Bernfield, P. 1995. Amylase α and β. Methods in enzymology, vol. 1, 149–158. New York: Academic Press.

    Google Scholar 

  • Bomba, A., R. Nemcoa, S. Gancarcíková, R. Herich, P. Guba, and D. Mudronová. 2002. Improvement of the probiotic effect of micro-organisms by their combination with maltodextrins, fructo-oligosaccharides and polyunsaturated fatty acids. British Journal of Nutrition 88: 95–99.

  • Boonthai, T., V. Vuthhiphandchai, and S. Nimrat. 2011. Probiotic bacteria effects on growth and bacterial composition of black tiger shrimp (Penaus monodon). Aquaculture Nutrition. doi:10.1111/j.1365-2095.2011.00865.x.

    Google Scholar 

  • Chow, S.N., and P.A. Sandifer. 1991. Differences in growth, morphometric traits and male sexual maturity among pacific white shrimp, Penaeus vannamei, from different commercial hatcheries. Aquaculture 92: 165–178.

    Article  Google Scholar 

  • Cobb, B.F., F.S. Conte, and M.A. Edwards. 1975. Free amino acids and osmoregulation in penaeid shrimp. Journal of Agricultural Food Chemistry 23: 1172–1174.

  • Deeseenthum, S., V. Leelavatcharams, and D.J. Brook. 2007. Effects of feeding Baciilus spp. as probiotic bacteria on growth of giant freshwater prawn (Macrobrachium rosenbegii De man). Pakistan Journal of Biological Sciences 10: 1481–1485.

    Article  PubMed  Google Scholar 

  • Deshimaru, O., and K. Shigeno. 1972. Introduction to the artificial diet for prawn Penaeus japonicus. Aquaculture 1: 115–133.

  • Fang, L.S., C.K. Tang, D.L. Lee, and I.M. Chen. 1992. Free amino acid composition in muscle and hemolymph of the prawn Penaeusmonodon in different salinities. Nippon Suisan Gakkaishi 58: 1095–1102.

  • Folch, J., M. Lees, and G.H. Bloane-Stanley. 1957. A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 266: 497–509.

    Google Scholar 

  • Furne, M., M.C. Hidalgo, A. Lopez, M. Garcia-Gallego, A.E. Morales, A. Domenzain, J. Domezain, and A. Sanz. 2005. Digestive enzyme activities in Adriatic sturgeon Acipenser naccarii and rainbow trout Oncorhynchus mykiss. A comparative study. Aquaculture 250: 391–398.

    Article  CAS  Google Scholar 

  • Gade, D., and M.K. Grieshaber. 1986. Pyruvate reductases catalyzes the formation of lactate and opines in anaerobic invertebrates. Comparative Biochemistry and Physiology 83b: 255–272.

  • Ghosh, S., A. Sinha, and C. Sahu. 2007. Effect of probiotic on reproductive performance in female live bearing ornamental fish. Aquaculture Research 38: 518–526.

    Article  Google Scholar 

  • Gomez, R., D. Geovanny, and M.A. Shen. 2008. Influence of probiotics on the growth and digestive enzyme activity of white pacific shrimp (Litopenaeus vannamei). Journal of Ocean University of China 7: 2.

    Article  Google Scholar 

  • Heizhao, Z.L., G. Zhixun, Y. Yingying, Z. Wenhu, and J.L. Zhuojia. 2004. Effect of dietary probiotics on apparent digestibility coefficients of nutrients of white shrimp, Litopenaeus vannamei Boon. Aquaculture Research 35: 1441–1447.

    Article  Google Scholar 

  • Hess, B., and J. Sherma. 2004. Quantification of arginine in dietary supplement tablets and capsules by silica gel high-performance thin-layer chromatography with visible mode densitometry. Acta Chromatographica 14: 60–69.

  • Hisano, H., R.D. Falcon, M. Maria Barrose, and E.L. Pezzato. 2008. Influence of yeast and yeast derivatives on growth performance and survival of Juvenile Prawn Macrobrachium amazonicum. Ciencia Animal Brasileira 9: 657–662.

    Google Scholar 

  • Holzapfel, W.H., P. Haberer, J. Snel, U. Schillinger, and J. Huisint Veld. 1998. Overview of gut flora and probiotics. International Journal of Food Microbiology 41: 85–101.

    Article  CAS  PubMed  Google Scholar 

  • Keysami, M.A., C.R. Saad, K. Sijam, H.M. Daud, and A.R. Alimon. 2007. Effect of Bacillus subtilis on growth development and survival of postlarvae Macrobrachium rosenbergii (de Man). Aquaculture Nutrition 13: 131–136.

  • Le Moullac, G., B. Klein, D. Sellos, and A. Van Wormhoudt. 1996. Adaptation of trypsin, chymotrypsin and alpha-amylase to casein level and protein source in Penaeus vannamei (Crustacea: Decapoda). Journal of Experimental Marine Biology and Ecology 208: 107–125.

  • Lee, P.G., and A.L. Lawrence. 1997. Digestibility. In Crustacean nutrition. Advances in world aquaculture, vol. 6, ed. L.R. D’Abramo, D.E. Conklin, and D.M. Akiyama, 194–260. Baton Rouge: World Aquaculture Society.

    Google Scholar 

  • Lovett D.L., and D.L. Felder 1990. Ontogenic change in digestive enzyme activity of larval and postlarval white shrimp Penaeus setiferus (Crustacea, Decapoda, Penaeidae). Biology Bulletin 178: 144–159.

  • Lowry, O.H., W.J. Rosenbrough, A.L. Fair, and R.J. Randall. 1951. Protein measurement with the folin phenol reagent. The Journal of Biological Chemistry 193: 265–275.

    CAS  PubMed  Google Scholar 

  • New, M.B., and W.C. Valenti. 2000. Freshwater prawn culture: the farming of Macrobrachium rosenbergii. Oxford: Blackwell Science.

    Book  Google Scholar 

  • Panigrahi, A., and I.S. Azad. 2007. Microbial intervention for better fish health in aquaculture: The Indian scenario. Fish Physiology and Biochemistry 33: 429–440.

    Article  CAS  Google Scholar 

  • Peixoto, S., R. Soares, W. Wasielesky, R.A. Cavalli, and L. Jensen. 2004. Morphometric relationship of length and weight of cultured Farfantepenaeus paulensis during nursery, grow out, and brood stock production phases. Aquaculture 242: 292–299.

    Google Scholar 

  • Prasad, L., B.B. Nayak, M.P.S. Kohli, A.K. Reddy, and P.P. Srivastava. 2012. Effect of feed supplemented exogenous bacteria, Lactobacillus sporogenes on growth performance of post larvae of Macrobrachium rosenbergii (de Man). Israeli Journal of Aquaculture 64: 676.

    Google Scholar 

  • Prasad, L., B.B. Nayak, P.P. Srivastava, A.K. Reddy, and M.P.S. Kohli. 2013. Use of Brewer’s Yeast Saccharomyces cerevisiae as growth promoter in giant freshwater prawn (Macrobrachium rosenbergii De man) post larvae. Turkish Journal of Fisheries and Aquatic Science 13: 447–452.

  • Rinisha, K., K.M. Mujeeb Rahiman, M. Razia Beevi, A.P. Thomas, and A.A. Mohamed Hatha. 2010. Probiotic effects of Bacillus spp. on the growth and survival of postlarvae of Macrobrachium rosenbergii. Fish Technologies 47: 173–178.

  • Roe, J.H. 1955. The determination of sugar and blood and spinal fluid with anthrone reagent. Journal of Biological Chemistry 212: 335–343.

    CAS  PubMed  Google Scholar 

  • Saad, S.A., M.M. Habashy, and M.K. Sharshar. 2009. Growth response of the freshwater prawn, Macrobrachium rosenbergii (De Man), to diets having different levels of Biogen®. World Applied Sciences Journal 6: 550–556.

    CAS  Google Scholar 

  • Seenivasan, C., P.S. Bhavan, S. Radhakrishnan, T. Muralisankar, G. Immanuel, V. Srinivasan, and N. Manickam. 2013. Effect of Saccharomyces cerevisiae on survival, growth, biochemical constituents and energy utilization in the prawn Macrobrachium rosenbergii. International Journal of Applied Biology and Pharmaceutical Technology 4: 39–47.

    Google Scholar 

  • Seenivasan, C., P.S. Bhavan, S. Radhakrishnan, and R. Shanthi. 2012a. Enrichment of Artemia nauplii with Lactobacillus sporogenes for enhancing the survival, growth and levels of biochemical constituents in the post-larvae of the freshwater prawn Macrobrachium rosenbergii. Turkish Journal of Fisheries and Aquatic Science 12: 23–31.

    Google Scholar 

  • Seenivasan, C., P.S. Bhavan, S. Radhakrishnan, and T. Muralisankar. 2012b. Effects of Probiotics on survival, growth and biochemical characteristics of freshwater prawn Macrobrachium rosenbergii Post Larvae. Turkish Journal of Fisheries and Aquatic Science 12: 331–338.

    Google Scholar 

  • Seenivasan, C., S. Radhakrishnan, T. Muralisankar, and P.S. Bhavan. 2012c. Influence of combined probiotics Lactobacillus sporogenes and Bacillus subtilis on survival, growth, biochemical changes and energy utilization performance of Macrobrachium rosenbergii (De Man 1879) post larvae. Journal of Ecobiotechnology 4: 29–34.

    CAS  Google Scholar 

  • Seenivasan, C., S. Radhakrishnan, T. Muralisankar, and P.S. Bhavan. 2012d. Bacillus subtilis on survival, growth, biochemical constituents and energy utilization of the freshwater prawn Macrobrachium rosenbergii post larvae. Egyptian Journal of Aquatic Research 38: 195–203.

    Article  Google Scholar 

  • Seenivasan, C., S. Radhakrishnan, T. Muralisankar, and P.S. Bhavan. 2012e. Efficacy of probiotics on survival, growth, biochemical changes and energy utilization performance of Macrobrachium rosenbergii (De Man 1879) post-larvae. Journal of Scientific Research 4(3): 729–740.

    Article  Google Scholar 

  • Seenivasan, C., P.S. Bhavan, and S. Radhakrishnan. 2011. Effect of probiotics (Binifit™) on survival, growth, biochemical constituents and energy budget of the freshwater prawn Macrobrachium rosenbergii post larvae. Elixir Aquaculture 41: 5919–5927.

    Google Scholar 

  • Shen, T., and J.Y. Wang. 1990. Biochemistry. (M). 67–86. Higher education publisher.

  • Shinde, A.N., V.B. Mulye, N.D. Chogale, V.R. Bhatkar, R.D. Bondre, A.S. Mohite. 2008. Effect of different probiotics Macrobrachium rosenbergii (De-Man) post larvae. Aquaculture 9: 7–12.

  • Sissons, J.W. 1989. Potential of probiotic organisms to prevent diarrhoea and promote digestion in farm animals. Journal of Science Food and Agriculture 49: 1–13.

    Article  Google Scholar 

  • Stechmiller, J.K., B. Langkamp-Henken, B. Childress, K.A. Herrlinger-Garcia, J. Hudgens, and L. Tian. 2005. Arginine supplementation does not enhance serum nitric oxide levels in elderly nursing home residents with pressure ulcers. Biological research for nursing 6: 289–299.

  • Suzer, C., D. Coban, O.H. Kamaci, S. Saka, K. Firat, O. Otgucuoglu, and H. Kucuksari. 2008. Lactobacillus spp. bacteria as probiotics in gilthead sea bream (Sparus aurata, L.) larvae: Effects on growth performance and digestive enzyme activities. Aquaculture 280: 140–145.

    Article  CAS  Google Scholar 

  • Taoka, Y., H. Maeda, J.Y. Jo, M.J. Jeon, S.C. Bai, W.J. Lee, K. Yuge, and S. Koshio. 2006. Growth, stress tolerance and nonspecific immune response of Japanese flounder Paralichthys olivaceus to probiotics in a closed recirculating system. Fisheries Science 72: 310–321.

    Article  CAS  Google Scholar 

  • Tapiero, H., G. Mathe, P. Couvreur, and K.D. Tew. 2002. Glutamine and glutamate. Biomedicine and Pharmacotherapy 56: 446–457.

  • Thompson, A.B., A.S. McGill, J. Murray, R. Hardy, and P.F. Howgate. 1980. The analysis of a range of non-volatile constituents of cooked haddock (Gadus aeglefinus) and the influence of these on flavor. In Advances in fish science and technology, ed. J.J. Connell, 484. Farnham, Surrey: Fishing Books.

  • Torres, J.J., B.W. Belman, and J.J. Childress. 1979. Oxygen consumption rates of midwater fishes as a function of depth of occurrence. Deep Sea Research 26A: 185–197.

  • Van Wormhoudt, A., and P. Favrel. 1988. Electrophoretic characterization of Palaemonelegans (Crustacea: Decapoda) amylase system: Study of amylase polymorphism during the intermolt cycle. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 89: 201–207.

  • Venkat, H.K., N.P. Sahu, and K.K. Jain. 2004. Effect of feeding Lactobacillus based probiotics on the gut microflora, growth and survival of postlarvae of Macrobrachium rosenbergii (de Man). Aquaculture Research 35: 501–507.

    Article  Google Scholar 

  • Verschuere, L., G. Rombaut, P. Sorgeloos, and W. Verstraete. 2000. Probiotic bacteria as biological control agents in aquaculture. Microbiology and Molecular Biology Reviews 64: 655–671.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Vine, N.G., W.D. Leukes, H. Kaiser, S. Daya, J. Baxter, and T. Hecht. 2004.Competition for attachment of aquaculture candidate probiotic and pathogenic bacteria on fish intestinal mucus. Journal of Fish Diseases 27: 319–326.

  • Wang, Y.B., Z.Q. Tian, J.T. Yao, and W.F. Li. 2008. Effect of probiotics, Enteroccus faecium, on tilapia (Oreochromis niloticus) growth performance and immune response. Aquaculture 277: 203–207.

    Article  Google Scholar 

  • Wang, Y.B., and Z.R. Xu. 2006. Effect of probiotics for common carp (Cyprinus carpio) based on growth performance and digestive enzyme activities. Animal Feed Science and Technology 127: 283–292.

    Article  CAS  Google Scholar 

  • Wang, Y.B., Z.R. Xu, and M.S. Xia. 2005. The effectiveness of commercial probiotics in Northern White Shrimp (Penaeus vannamei L.) ponds. Fisheries Science 71: 1034–1039.

    Google Scholar 

  • Wilson, R.P. 1989. Amino acids and proteins. In Fish nutrition, J.E. Halver, 11–151. 2nd ed. New York: Academic Press.

  • Wilson, R.P. 2002. Amino acids and protein. In Fish nutrition, ed. J.E. Halver, and R.W. Hardy, 143–179. San Diego, CA, USA: Academic Press.

  • Witte, M.B., and A. Barbul. 2003. Arginine physiology and its implication for wound healing. Wound Repair and Regeneration, 11: 419–423.

  • Yanbo, W., and X. Zirong. 2006. Effect of probiotics for common carp (Cyprinus carpio) based on growth performance and digestive enzyme activities. Animal Feed Science and Technology 127: 283–292.

    Article  Google Scholar 

  • Ziaei-Nejad, S., M.H. Rezaei, G.A. Takami, D.L. Lovett, A.R. Mirvaghefi, and M. Shakouri. 2006. The effect of Bacillus spp. bacteria used as probiotics on digestive enzyme activity, survival and growth in the Indian white shrimp Fenneropenaeus indicus. Aquaculture 252: 516–524.

    Article  CAS  Google Scholar 

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Acknowledgments

The Bharathiar University, Coimbatore, Tamilnadu, India is gratefully acknowledged for the financial support provided in the form of University Research Fellowship to Mr. C. Seenivasan. We thank Dr. G. Immanuel, Centre for Marine Sciences and Technology (Rajakkamangalam, Nagar Kovil-629502), Manonmaniam Sundaranar University, Thirunelveli, Tamilnadu, India for providing subject expertise in conducting experiments.

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  1. Crustacean Biology Laboratory, Department of Zoology, Bharathiar University, Coimbatore, 641 046, Tamil Nadu, India

    C. Seenivasan, S. Radhakrishnan, T. Muralisankar & P. Saravana Bhavan

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  1. C. Seenivasan
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Seenivasan, C., Radhakrishnan, S., Muralisankar, T. et al. Effects of Probiotics on Survival, Growth and Digestive Enzymes Activities in Freshwater Prawn Macrobrachium rosenbergii (De Man 1879). Proc Zool Soc 69, 52–60 (2016). https://doi.org/10.1007/s12595-014-0123-6

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