Abstract
Peroxiredoxins (Prx) are ubiquitous, highly conserved peroxidases whose activity depends on catalytic cysteine residues. The Prx1-class of the peroxiredoxin family, also called typical 2-Cys Prx, organize as head-to-tail homodimers containing two active sites. The peroxidatic cysteine CP of one monomer reacts with the peroxide substrate to form sulfenic acid that reacts with the resolving cysteine (CR) of the adjacent subunit to form an intermolecular disulfide, that is reduced back by the thioredoxin/thioredoxin reductase/NADPH system. Although the minimal catalytic unit is the dimer, these Prx oligomerize into (do)decamers. In addition, these ring-shaped decamers can pile-up into high molecular weight structures. Prx not only display peroxidase activity reducing H2O2, peroxynitrous acid and lipid hydroperoxides (antioxidant enzymes), but also exhibit holdase activity protecting other proteins from unfolding (molecular chaperones). Highly relevant is their participation in redox cellular signaling that is currently under active investigation. The different activities attributed to Prx are strongly ligated to their quaternary structure. In this review, we will describe different biophysical approaches used to characterize the oligomerization dynamics of Prx that include the classical size-exclusion chromatography, analytical ultracentrifugation, calorimetry, and also fluorescence anisotropy and lifetime measurements, as well as mass photometry.
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Funding
This work was supported by grants from Universidad de la República CSIC I + D Grupos 46725 to A.D., CSIC I + D to M.N.M. and CSIC iniciación to S.F.V.
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Conceptualization, A.D.; funding acquisition, S.F.V., M.N.M. and A.D.; visualization, S.F.V. and M.N.M.; writing – original draft, A.D.; writing – review & editing, S.F.V., M.N.M., and A.D. MNM and AD contributed equally to the writing and revision of this review. S.F.V. created all the figures. All authors have given approval to the final version of the manuscript.
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Villar, S.F., Möller, M.N. & Denicola, A. Biophysical tools to study the oligomerization dynamics of Prx1-class peroxiredoxins. Biophys Rev 15, 601–609 (2023). https://doi.org/10.1007/s12551-023-01076-3
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DOI: https://doi.org/10.1007/s12551-023-01076-3