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Extended-spectrum beta-lactamase-producing Klebsiella pneumoniae infection in a neonatal intensive care unit

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Abstract

Background

A molecular epidemiological survey was conducted on an extended-spectrum beta-lactamase-producing Klebsiella pneumoniae (ESBLKp) infection in our neonatal intensive care unit (NICU) from February to June 2008.

Methods

Cultures of clinical samples from neonates in the NICU, the hands of healthcare workers and the environment of the NICU were subjected to ESBLKp isolation. Pulsed-field gel electrophoresis was performed to determine Klebsiella pneumoniae strains (type A-D).

Results

In 1439 neonates, 38 (2.6%) had infections and 65 (4.5%) had colonizations with ESBLKp. Microbiological sampling of the NICU environment yielded 33 (14.9%) ESBLKp isolates from 222 samples. Clone A was found in 88.2% of the infected neonates, 66.7% of the colonized neonates, 69.7% of the environmental samples, and the hands of a healthcare worker.

Conclusions

The detection rate of ESBLKp is high in environmental samples, especially those from frequently touched surfaces. Since ESBLKp was identified on the hands of a healthcare worker in the present study, hand and environmental hygiene is mandatory for infection control in neonatal intensive care units.

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Authors and Affiliations

  1. Department of Infection Control, Women’s Hospital, Zhejiang University School of Medicine, Hangzhou, 310006, China

    Rong Lin, Bo Wu, Xin-Fen Xu, Xin-Chang Liu, Hong Ye & Guang-Yong Ye

  2. Women’s Hospital, Zhejiang University School of Medicine, Hangzhou, 310006, China

    Xin-Fen Xu

Authors
  1. Rong Lin
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  2. Bo Wu
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  3. Xin-Fen Xu
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  4. Xin-Chang Liu
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  5. Hong Ye
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  6. Guang-Yong Ye
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Correspondence to Xin-Fen Xu.

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Lin, R., Wu, B., Xu, XF. et al. Extended-spectrum beta-lactamase-producing Klebsiella pneumoniae infection in a neonatal intensive care unit. World J Pediatr 8, 268–271 (2012). https://doi.org/10.1007/s12519-012-0370-4

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  • DOI: https://doi.org/10.1007/s12519-012-0370-4

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