Abstract
Streak mosaic is a new disease of sugarcane in Indonesia caused by Sugarcane streak mosaic virus (SCSMV). An extensive survey conducted during milling season 2008/2009 at 30 sugar factories (SF) across the Java revealed that about 30 % of observed sugarcane fields of 28 SF were affected by the streak mosaic disease. Most commercial cane cultivars were infected by the virus but the cultivar PS 864 was found most susceptible. RT-PCR detection, using SCSMV coat protein specific gene primers SCSMV-cpF and SCSMV AP3, successfully amplified a 500 bp DNA fragment, suggesting the positive identity of the SCSMV with all the tested symptomatic samples. Protein analysis of the virus confirmed that SCSMV has a coat protein of size approximately 40 kDa and flexuous, filamentous particles about 890 nm in length was observed under an electron microscope. The virus was easily transmitted by infected cane cuttings and mechanically by sap inoculation and cutting knife. Host range test on 23 plant species revealed that maize, sorghum and Dactyloctenium aegyptium were alternative hosts of SCSMV. A preliminary yield loss assessment on PS 864 cultivar revealed that the disease incidence at ≥50 % reduced sugar yield by about 20 %. Hot water treatment of cane cuttings was not able to eliminate the virus in cane stalks but only postponed the appearance of the symptom. Response of 16 commercial cane cultivars to artificially inoculation of SCSMV using an abrasive pad rubbing technique showed that only five cultivars were resistant to the disease.
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References
Anon. 2010. Indonesia Sugar Research Institute, Annual Report 2009. (in Indonesian).
Anon. 2011. Revitalization of Stated Own Sugar Industry 2010–2014, the Indonesian Ministry of State Own Company. (in Indonesian).
Balamuralikrishnan, M., S. Doraisamy, T. Ganapathy, and R. Viswanathan. 2003. Impact of serial thermotherapy on Sugarcane mosaic virus titre and regeneration in sugarcane. Archives of Phytopathology and Plant Protection 36: 173–178.
Chatenet, M., C. Mazarin, and J. Girard. 2005. Detection of Sugarcane streak mosaic virus in sugarcane from several Asian countries. Sugar Cane International 23(4): 12–15.
Hadidi, A., R.K. Khetarpal, and H. Koganezawa. 1998. Plant virus disease control. St. Paul: APS Press.
Hall, J.S., B. Adams, T.J. Parsons, R. Frech, L.C. Lane, and S.G. Jensen. 1998. Molecular cloning, sequencing, and phylogenetic relationships of a new potyvirus: Sugarcane streak mosaic virus, and a reevaluation of classification of the Potyviridae. Molecular Phylogenetic and Evolution 10: 323–332.
Handojo, H., Siswojo, and L. Legowo. 1978. Mosaic disease, yield losses and resistance testing. Majalah Perusahaan Gula 17: 1–5. (in Indonesian).
Hema, M., J. Joseph, K. Gopinath, P. Sreenivasulu, and H.S. Savithri. 1999. Molecular characterization and interviral relatiomships of flexuous virus causing mosaic disease of sugarcane (Saccharum officinarum L.) in India. Archives of Virology 144: 479–490.
Hema, M., H.S. Savithri, and P. Sreenivasulu. 2003. Comparison of direct binding polymerase chain reaction with recombinant coat protein antibody based dot-blot immunobinding assay and immunocapture-reverse transcription-polymerase chain reaction for the detection of Sugarcane streak mosaic virus causing mosaic disease of sugarcane in India. Current Science 85(12): 1774–1777.
Kristini, A., Irawan, and D. Sasongko. 2006. Mosaic Booming. Gula Indonesia 30(1): 36–38. (in Indonesian).
Li, W., Z. He, and S. Li. 2011. Molecular characterization of a new strain of Sugarcane streak mosaic virus (SCSMV). Archives of Virology 156: 2101–2104.
Putra, L.K., H.J. Ogle, A.P. James, and P.J.L. Whittle. 2003. Distribution of Sugarcane mosaic virus in sugarcane plants. Australasian Plant Pathology 32: 305–307.
Putra, L.K., and T.A. Damayanti. 2009. Streak mosaic disease of sugarcane in Indonesia: field survey, detection, transmission test, host range and varietal resistance. Majalah Penelitian Gula 45(1): 21–38. (in Indonesian).
Srisink, S., P.W.J. Taylor, J.K. Stringer, and D.S. Teakle. 1994. An abrasive pad rubbing method for inoculating sugarcane with sugarcane mosaic virus. Australian Journal of Agricultural Research 45: 625–631.
Viswanathan, R., and G.P. Rao. 2011. Disease scenario and management of major sugarcane disease in India. Sugar Tech 13(4): 336–353.
Acknowledgments
This research was financially supported by the Indonesian Ministry of Agriculture through KKP3T research program and the Australian Centre for International Agricultural Research through HORT/206/147 research project. The authors wish to thank Dr. Rob Magarey (Project Leader of HORT/206/147) for his guidance and support during implementation of the project. We also thank Mr. Edi Supardi, Mr. Abd. Hafid Asnawi, Mr. Herwan Cahyono Adi, Mr. Tojib, Mr. Edi Sanyoto, Mr. Mustajab and Mr. M. Sochieb for excellent support during the research.
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Putra, L.K., Kristini, A., Achadian, E.M. et al. Sugarcane streak mosaic virus in Indonesia: Distribution, Characterisation, Yield Losses and Management Approaches. Sugar Tech 16, 392–399 (2014). https://doi.org/10.1007/s12355-013-0279-9
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DOI: https://doi.org/10.1007/s12355-013-0279-9