Abstract
Abiotic stress caused by unsuitable environmental changes brings serious impacts on the growth and development of sorghum, resulting in significant loss in yield and quality every year. Phospholipase D is one of the key enzymes that catalyze the hydrolysis of phospholipids, and participates in plants response to abiotic stresses and phytohormones, whereas as the main producers of Phosphatidic acid (PA) signal, the detailed information about Phospholipase D associated (SbPLD) family in sorghum has been rarely reported. This study was performed to identify the PLD family gene in sorghum based on the latest genome annotation and to determine the expression of PLDs under abiotic stresses by qRT-PCR analysis. In this study, 13 PLD genes were identified in sorghum genome and further divided into 7 groups according to the phylogenetic analysis. All sorghum PLD family members harbored two conserved domains (HDK1&2) with catalytic activity, and most members contained a C2 domain. In ζ subfamily, C2 domain was replaced by PX and PH domain. The exon–intron structure of SbPLD genes within the same subfamily was highly conservative. The tissue specific expression analysis revealed different expression of SbPLD genes in various developmental stages. High level expression of SbPLDα3 was observed in almost all tissues, whereas SbPLDα4 was mainly expressed in roots. Under abiotic stress conditions, SbPLD genes responded actively to NaCl, ABA, drought (PEG) and cold (4 °C) treatment at the transcriptional level. The expression of SbPLDβ1 was significantly up-regulated, while the transcription of SbPLDζ was suppressed under various stress conditions. In addition, SbPLDβ1 and SbPLDδ2 were predicted to be the target genes of sbi-miR159 and sbi-miR167, respectively. This study will help to decipher the roles of PLDs in sorghum growth and abiotic stress responses.
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This work was supported by grants from the Natural Science Foundation of Heilongjiang Province (ZD2020C007); National key research and development program of China (2016YFD0101002); Program of cultivation on campus of Heilongjiang Bayi Agricultural University (XZR2014-02).
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JW, WS and XL collected and analyzed the data, CZ and LH prepared visualization of data, JW and WS prepared the manuscript for publication, GY and JX designed the study and reviewed the draft of the manuscript. All authors have read and agreed to the published version of the manuscript.
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Wei, J., Shao, W., Liu, X. et al. Genome-wide identification and expression analysis of phospholipase D gene in leaves of sorghum in response to abiotic stresses. Physiol Mol Biol Plants 28, 1261–1276 (2022). https://doi.org/10.1007/s12298-022-01200-9
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DOI: https://doi.org/10.1007/s12298-022-01200-9