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Identification of GH17 gene family in Vitis vinifera and expression analysis of GH17 under various adversities

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Abstract

Glycoside hydrolase (GH, EC 3.2.1) is a group of enzymes that hydrolyzes glycosidic bonds and play a role in the hydrolysis and synthesis of sugars in living organisms. Vitis vinifera is an important fruit crop and it harbors GH17 gene family however, their function in grapes has not been systematically investigated. In this study, a total of 870 GH17 genes were identified from 14 plant species and their structural domain, sequence alignment, phylogenetic tree, collinear analysis, with the expression profiles of VviGH17 gene family was performed. The promoter analysis of VviGH17 gene showed the presence of cis-acting elements, which are responsive to plant growth and development. In addition, elements for plant hormones were found that are triggered in response to abiotic/biological stress. Transcriptomic data led to the identification of several VviGH17 genes, which are associated with bud dormancy and in response to abiotic stress. Transcript analysis was carried out for some of the selected VviGH17 genes RT-qPCR. VviGH17-16 and VviGH17-30 genes were differentially expressed during bud dormancy, fruit development and different abiotic stresses. Moreover, VviGH17-37 and VviGH17-44 were differentially expressed at fruit development, in response to abiotic stress. In addition, subcellular localization predicts that the VviGH17-16, VviGH17-30, and VviGH17-37 genes were located in the cell membrane, while VviGH17-44 gene was located in the vacuole. In conclusion, our study led to the identification of several GH17s and their probable role in development and stress.

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Funding

This project was funded by the General Program of National Natural Science Foundation of China (31772283), Natural Science Foundation of Jiangsu Province (BK20201321), Jiangsu Planned Projects for Postdoctoral Research Funds (2020Z052), Jiangsu Agriculture Science and Technology Innovation Fund (CX(20)3016), and A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.

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  1. Tariq Pervaiz and Tianhua Liu have contributed equally to this work.

Authors and Affiliations

  1. Department of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu Province, 210095, People’s Republic of China

    Tariq Pervaiz, Tianhua Liu, Xiang Fang, Yanhua Ren, Xiyang Li, Zhongjie Liu, Muhammad Fiaz, Jinggui Fang & Lingfei Shangguan

  2. Fruit Crop Genetic Improvement and Seedling Propagation Engineering, Center of Jiangsu Province, Nanjing, 210095, People’s Republic of China

    Tianhua Liu, Yanhua Ren, Xiyang Li, Zhongjie Liu, Muhammad Fiaz, Jinggui Fang & Lingfei Shangguan

  3. Beijing Advanced Innovation Center for Tree Breeding By Molecular Design, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, People’s Republic of China

    Tariq Pervaiz

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  1. Tariq Pervaiz
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Contributions

Conceived and designed the experiments: LFSG, JGF. Analyzed the data: LFSG, THL, XF, YHR, ZJL. RT-qPCR: XYL, MF. Wrote the paper: LFSG, THL, TP. Revised the paper: LFSG, TP. TP and TL conduct experimentation and drafting, LS, XF, Y R and XL retrieve and data analysis, ZL, MF and JF revised the manuscript and Technical input.

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Correspondence to Lingfei Shangguan.

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Pervaiz, T., Liu, T., Fang, X. et al. Identification of GH17 gene family in Vitis vinifera and expression analysis of GH17 under various adversities. Physiol Mol Biol Plants 27, 1423–1436 (2021). https://doi.org/10.1007/s12298-021-01014-1

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