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Pre-treatment of two contrasting water-stressed genotypes of cassava (Manihot esculenta Crantz) with ascorbic acid. I. Growth, physiological and antioxidant responses

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Abstract

Water deficit-stress at early growth stage is a major constraint of cassava production. Ascorbic acid is a non-enzymatic antioxidant that mitigates oxidative damage caused by water stress in plants. Growth, physiological and antioxidant defense system responses of two contrasting water-stressed cassava genotypes to pre-treatment with foliar application of ascorbic acid (AA) were investigated. The objectives of this study were to assess the growth, proline, photosynthesis pigments and antioxidant activities of young water-stressed cassava plants pre-treated with ascorbic acid. The study consisted of IITA-TMS-IBA980581 (drought tolerant) and IITA-TMS-IBA010040 (drought sensitive) cassava genotypes treated with six doses (0.00, 0.25, 0.50, 0.75 and 1.00 mM) of AA before being subjected to water deficit (45.0% field capacity) and a water sufficient AA-untreated control. In both genotypes, water stress reduced shoot height (40.3%), leaf area (42.5%), and number of root (54.5%), biomass (28.6%), relative water content (RWC, 3.2%) and photosynthetic pigments (300.0%). However, water stress increased proline (91.3%), endogenous AA (112.0%), catalase (CAT, 300.0%) and superoxide dismutase (SOD, 15.3%) in both genotypes. Compared with IITA-TMS-IBA010040, leaf area, biomass, number of root and shoot height of IITA-TMS-IBA980581 were higher by 7.3, 24.6, 25.9 and 13.1%, respectively. By less than a quarter, chlorophylls a and b, activity of superoxide dismutase and relative water content of IITA-TMS-IBA980581 were higher compared with IITA-TMS-IBA010040. However, proline content of IITA-TMS-IBA010040 was higher than IITA-TMS-IBA980581 by 14.3%. Pre-treatment with AA improved growth parameters, photosynthetic pigments, RWC, endogenous AA, activity of CAT and SOD, but decreased proline in both genotypes with an optimum concentration at 0.5 mM. Pre-treatment with 0.5 mM AA increased shoot height, area of leaves, leaf number, number of root and dry weight by 46.3, 44.7, 14.4, 88.2 and 37.5%, respectively. Pre-treatment with 0.5 mM AA doubled chlorophylls, tripled carotenoids content, doubled endogenous AA and slightly enhanced RWC (2.1%) and SOD (2.0%) when compared with AA-untreated water stressed plants. But pre-stress application of AA reduced proline content by one-fold, increased CAT activity by one-fold in IITA-TMS-IBA980581 and by one-third in IITA-TMS-IBA010040. The study concluded that pre-treatment of cassava young plants with AA before water deficit could alleviate oxidative stress.

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Abbreviations

AA:

Ascorbic acid

ABA:

Abscisic acid

CAT:

Catalase

EDTA:

Ethylene diamine tetraacetic acid

ROS:

Reactive oxygen species

RWC:

Relative water content

SOD:

Superoxide dismutase

References

  • Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–126

    CAS  Google Scholar 

  • Ahmad I, Basra SMA, Wahid A (2014) Exogenous application of ascorbic acid, salicylic acid, hydrogen peroxide improves the productivity of hybrid maize at low temperature stress. Int J Agric Biol 16:825–830

    CAS  Google Scholar 

  • Ahmed F, Baloch DM, Sadiq SA, Ahmed SS, Hanan A, Taran SA (2014) Plant growth regulators induced drought tolerance in sunflower (Helianthus annuus L.) hybrids. J Anim Plant Sci 24:886–890

    CAS  Google Scholar 

  • Akram NA, Shafiq F, Ashraf M (2017) Ascorbic acid-a potential oxidant scavenger and its role in plant development and abiotic stress tolerance. Front Plant Sci 8:613. https://doi.org/10.3389/fpls.2017.00613

    Article  PubMed  PubMed Central  Google Scholar 

  • Amin B, Mahleghah G, Mahmood HMR, Hossein M (2009) Evaluation of interaction effect of drought stress with ascorbate and salicylic acid on some of physiological and biochemical parameters in okra (Hibiscus esculentus L.). Res J Biol Sci 4:380–387. http://medwelljournals.com/abstract/?doi=rjbsci.2009.380.387

    Google Scholar 

  • Anjum SA, Xie X, Wang L, Saleem MF, Man C, Lei W (2011) Morphological, physiological and biochemical responses of plants to drought stress. Afr J Agric Res 6:2026–2032

    Google Scholar 

  • Anwar J, Farooqi MI, Nagra SA, Khan AM (1989) A new method for spectrophotometric determination of ascorbic acid. J Chem Soc Pak 12(1):1990

    Google Scholar 

  • Ashraf M (2009) Biotechnological approach of improving plant salt tolerance using antioxidants as markers. Biotechnol Adv 27(1):84–93. https://doi.org/10.1016/j.biotechadv.2008.09.003

    Article  CAS  PubMed  Google Scholar 

  • Athar H, Khan A, Ashraf M (2008) Exogenously applied ascorbic acid alleviates salt induced oxidative stress in wheat. Environ Exp Bot 63:224–231. https://doi.org/10.1016/j.envexpbot.2007.10.018

    Article  CAS  Google Scholar 

  • Bakayoko S, Tschannen A, Nindjin C, Dao D, Girardin O, Assa A (2009) Impact of water stress on fresh tuber yield and dry matter content of cassava (Manihot esculenta Crantz) in Côte d’Ivoire. Afr J Agric Res 4:021–027

  • Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–207

    Article  CAS  Google Scholar 

  • De Faria AP, Lemos-Filho JP, Modolo LV, Franc FGC (2013) Electrolyte leakage and chlorophyll a fluorescence among castor bean cultivars under induced water deficit. Acta Physiol Plant 35:119–128

    Article  CAS  Google Scholar 

  • Dhindsa RS, Dhindsa PP, Thorpe TA (1980) Leaf senescence correlated with increased levels of membrane permeability and lipid-peroxidation and decreased levels of superoxide dismutase and catalase. J Exp Bot 32:93–101

    Article  Google Scholar 

  • Dolatabadian A, Sanavy SAMM, Asilan KS (2010) Effect of ascorbic acid foliar application on yield, yield component and several morphological traits of grain corn under water deficit stress conditions. Not Sci Biol 2:45–50. https://doi.org/10.15835/nsb234717

    Article  CAS  Google Scholar 

  • Duque LO, Setter TL (2013) Cassava response to water deficit in deep pots: root and shoot growth, ABA, and carbohydrate reserves in stems, leaves and storage roots. Trop Plant Biol 6:199–209

    Article  CAS  Google Scholar 

  • El-Sharkawy MA (2012) Stress-tolerant cassava: the role of integrative ecophysiology breeding research in crop improvement. Open J Soil Sci 2:162–186

    Article  Google Scholar 

  • Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 48:909–930

    Article  CAS  Google Scholar 

  • Hussein MM, Alva AK (2014) Effects of zinc and ascorbic acid application on the growth and photosynthetic pigments of millet plants grown under different salinity. Agric Sci 5:1253–1260. https://doi.org/10.4236/as.2014.513133

    Article  Google Scholar 

  • IPCC (2007) Climate change 2007. The physical science basis. In contribution of working group i to the fourth assessment report of the intergovernmental panel on climate change, 1009. Cambridge University Press, Cambridge

  • Khalil SE, Nahed G, Aziz A, Bedour LAH (2010) Effect of water stress and ascorbic acid on some morphological and biochemical composition of Ocimum basilicum plant. J Am Sci 6:33–46. https://doi.org/10.1007/s11738-014-1530-z

    Article  CAS  Google Scholar 

  • Latif M, Akram NA, Ashraf M (2016) Regulation of some biochemical attributes in drought-stressed cauliflower (Brassica oleracea L.) by seed pre-treatment with ascorbic acid. J Hort Sci Biotechnol 91:129–137. https://doi.org/10.1080/14620316.2015.1117226

    Article  CAS  Google Scholar 

  • Lichtenthaler HK, Wellburn AR (1985) Determination of total carotenoids and chlorophylls A and B of leaf in different solvents. Biol Soc Trans 11:591–592

    Article  Google Scholar 

  • Mazid M, Khan TA, Khan HZ, Quddusi S, Mohammad F (2011) Occurrence, biosynthesis and potentialities of ascorbic acid in plants. Int J Plant Anim Environ Sci 1:2231–4490

    Google Scholar 

  • Mittler R (2002) Oxidative stress, antioxidant and stress tolerance. Trends Plant Sci 7:405–410

    Article  CAS  Google Scholar 

  • Naz H, Akram NA, Ashraf M (2016) Impact of ascorbic acid on growth and some physiological attributes of cucumber (Cucumis sativus) plants under water-deficit conditions. Pak J Bot 48:877–883

    CAS  Google Scholar 

  • Ospina B, Ceballos H (2012) Cassava in the third millennium: modern production, processing, use and marketing systems. Published by CIAT/CLAYUCA/CTA, 574 PP

  • Pardales JR, Yamauchi A, Belmonte DV, Esquibel CB (2001) Dynamics of root development in root crops in relation to the prevailing moisture stress in the soil. In: Proceedings of the 6th symposium of the international society of root research, Nagoya, Japan, pp 72–73

  • Pereira FM, Zanetti S, Silva MA (2018) Water relations of cassava cultivated under water-deficit levels. Acta Physiol Plant 40:13. https://doi.org/10.1007/s11738-017-2590-7

    Article  Google Scholar 

  • Ray DK, Gerber JS, Macdonald GK, West PC (2015) Climate variation explains a third of global crop yield variability. Nat Commun 6:5989

    Article  CAS  Google Scholar 

  • Santisopasri V, Kurotjanawong K, Chotineeranat S, Piyachomkwan K, Sriroth K, Oates CG (2001) Impact of water stress on yield and quality of cassava starch. Ind Crops Prod 13:115–129. https://doi.org/10.1016/s0926-6690(00)00058-3

    Article  CAS  Google Scholar 

  • SAS (2003) Statistical analysis system, version 9.1. SAS Institute Inc., Cary

  • Shafiq S, Akram NA, Ashraf M, Arshad A (2014) Synergistic effects of drought and ascorbic acid on growth, mineral nutrients and oxidative defensesystem in canola (Brassica napus L.) plants. Acta Physiol Plant 36:1539–1553. https://doi.org/10.1007/s11738-014-1530-z

    Article  CAS  Google Scholar 

  • Silva MA, dos Jifon JL, Santos CM, Jadoski CJ, da Silva JAG (2013) Photosynthetic capacity and water use efficiency in sugarcane genotypes subject to water deficit during early growth phase. Braz Arch Biol and Technol 56(5):735–748

    Article  CAS  Google Scholar 

  • Sullivan CY, Ross WM (1979) Selecting the drought and heat resistance in grain sorghum. In: Mussel H, Stapes RC (eds) Stress physiology in crop plants. Wiley, New York, pp 263–281

    Google Scholar 

  • Szabo L, Savoure A (2010) Proline: a multifunctional amino acid. Trends Plant Sci 15(2):89–97

    Article  Google Scholar 

  • Turyagyenda LF, Kizito EB, Ferguson M, Baguma Y, Agaba M, Harvey JJW, Osiru DS (2013) Physiological andmolecular characterization of drought responses and identification of candidate tolerance genes in cassava. AoB Plants 5:7–24. https://doi.org/10.1093/aobpla/plt007

    Article  CAS  Google Scholar 

  • Vandegeer R, Miller RE, Bain M, Gleadow MR, Cavagnaro TR (2012) Drought adversely affects tuber development and nutritional quality of the staple crop cassava (Manihot esculenta Crantz). Funct Plant Biol 40:195–200. https://doi.org/10.1071/fp12179

    Article  Google Scholar 

  • Wedeking R, Mahlein A-K, Steiner U, Oerke E-C, Goldbach HE, Wimmer MA (2016) Osmotic adjustment of young sugar beets (Beta vulgaris) under progressive drought stress and subsequent rewatering assessed by metabolite analysis and infrared thermography. Funct Plant Biol. https://doi.org/10.1071/FP16112

    Article  Google Scholar 

  • Zhao P, Liu P, Shao J, Li C, Wang B, Guo X, Yan B, Xia Y, Peng M (2015) Analysis of different strategies adapted by two cassava cultivars in response to drought stress: ensuring survival or continuing growth. J Exp Bot 66:1477–1488

    Article  CAS  Google Scholar 

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Acknowledgements

The authors thank International Institute of Tropical Agriculture (IITA) for the donations of stem-cuttings of the two cassava genotypes used for the study. The authors appreciated the technical support of Professor E. Obuotor, Department of Biochemistry and Molecular Biology of the University.

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  1. Crop Production and Protection Department, Obafemi Awolowo University, Ile-Ife, Nigeria

    Omolara R. Ibrahim & Jelili T. Opabode

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  1. Omolara R. Ibrahim
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  2. Jelili T. Opabode
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Correspondence to Jelili T. Opabode.

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Ibrahim, O.R., Opabode, J.T. Pre-treatment of two contrasting water-stressed genotypes of cassava (Manihot esculenta Crantz) with ascorbic acid. I. Growth, physiological and antioxidant responses. Physiol Mol Biol Plants 25, 1385–1394 (2019). https://doi.org/10.1007/s12298-019-00709-w

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