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Mapping quantitative trait loci for heat tolerance at the booting stage using chromosomal segment substitution lines in rice

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Abstract

High temperature stress is a major obstacle in rice productivity. Considerable progress has been made on studying heat tolerance (HT) at different stages. However, the genetic basis of HT at the booting stage is poorly understood. In this study, we analyzed the morphological features of a heat-sensitive japonica cultivar Sasanishiki under natural high temperature stress at the booting stage. The anthers became smaller and the number, and fertility, of pollen grains were decreased significantly. As a result, there was a dramatic reduction in spikelet fertility. In contrast, the indica cultivar Habataki showed high HT and normal spikelet fertility under high temperature stress. Additonally, a set of chromosome segment substitution lines, derived from Sasanishiki and Habataki, were evaluated for HT related quantitative trait loci (QTLs) across two environments in the natural field. A total of 12 QTLs associated with HT were detected, of which, 5 were identified in two environments, and 7 in one environment. Furthermore, one of the major-effect QTLs (qHTB3-3) detected on the long arm of chromosome 3, was confirmed using overlapping substituted lines. qHTB3-3 was finally mapped between the two markers RM3525 and 3-M95, approximately 2.8 Mb apart. These findings and further gene cloning of qHTB3-3 will help us better understand the molecular control of HT in rice, and may contribute to the development of high HT rice varieties.

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Acknowledgements

This work was supported by the Key Program for Science and Technology of Jiangxi Province, China (20152ACF60015), and the Collaborative Innovation Program for Modern Agricultural Research of Jiangxi Province, China (JXXTCX2015001-005).

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Author notes

  1. Shan Zhu and Renliang Huang have contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, College of Agronomy, Jiangxi Agricultural University, Nanchang, 330045, China

    Shan Zhu & Haohua He

  2. Rice National Engineering Laboratory (Nanchang), Nanchang Sub-center, National Rice Improvement Center, Jiangxi Provincial Key Laboratory of Rice Physiology and Genetics, Rice Research Institute, Jiangxi Academy of Agricultural Sciences, Nanchang, 330200, China

    Shan Zhu, Renliang Huang, Hnin Pwint Wai, Hongliang Xiong, Xianhua Shen & Song Yan

  3. Biotechnology Research Department, Department of Research and Innovation, Ministry of Education (Ministry of Science and Technology), Kyaukse, 05151, Myanmar

    Hnin Pwint Wai

Authors
  1. Shan Zhu
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  2. Renliang Huang
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  4. Hongliang Xiong
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Correspondence to Haohua He or Song Yan.

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Zhu, S., Huang, R., Wai, H.P. et al. Mapping quantitative trait loci for heat tolerance at the booting stage using chromosomal segment substitution lines in rice. Physiol Mol Biol Plants 23, 817–825 (2017). https://doi.org/10.1007/s12298-017-0465-4

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  • DOI: https://doi.org/10.1007/s12298-017-0465-4

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