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Regeneration and assessment of genetic fidelity of the endangered tree Moringa peregrina (Forsk.) Fiori using Inter Simple Sequence Repeat (ISSR)

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Abstract

Moringa peregrina is an endangered species of Moringaceae. M. peregrina is a multipurpose tree with a wide variety of potential uses including its medicinal activity. In our study, a rapid and efficient micropropagation protocol for M. peregrina has been established. In vitro germinated seedlings were cultured on Murashige and Skoog (MS) medium supplemented with different levels of either 6-benzyladenine (BA) or kinetin (Kin). The maximum shoot proliferation of 6.5 shoots per explant with 100 % shoot proliferation rate was observed on MS medium supplemented with 1.0 mg/l BA. On the other hand, MS medium supplemented with 1 mg/l indole-3-butyric acid (IBA) resulted in the maximum number of roots. Micropropagated plants were successfully acclimatized. Genetic stability of micropropagated plants was assessed using Inter-Simple Sequence Repeat (ISSR). The amplification products were monomorphic in all in vitro grown plants. No polymorphism was detected indicating the genetic integrity of in vitro propagated plants. This micropropagation protocol could be useful for raising genetically uniform plants for plant propagation and commercial cultivation.

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References

  • Abdellatef E, Khalafalla M (2010) in vitro morphogenesis studies on Moringa olifera L. An important medicinal tree. Int J Medicobiol Res 1:85–89

    Google Scholar 

  • Bhatia R, Singh KP, Sharma TR, Jhang T (2011) Evaluation of the genetic fidelity of in vitro-propagated gerbera (Gerbera jamesonii Bolus) using DNA-based markers. Plant Cell Tissue Organ Cult 104:131–135

    Article  Google Scholar 

  • Carvalho LC, Goulo L, Oliveira C, Gonsalves JC, Amncio S (2004) RAPD assessment for identification of clonal identity and genetic stability of in vitro propagated chestnut hybrids. Plant Cell Tissue Organ Cult 77:23–27

    Article  CAS  Google Scholar 

  • Epstein E, Luduig-Muller J (1993) Indole-3-butyric acid in plants: occurrence, synthesis, metabolism and transport. Physiol Plant 88:382–389

    Article  CAS  Google Scholar 

  • Frabetti M, Gutiérrez-Pesce P, Mendoza-de Gyves E, Rugini E (2009) Micropropagation of Teucrium fruticans L., an ornamental and medicinal plant. In vitro Cell Dev Biol Plant 45:129–134

    Article  CAS  Google Scholar 

  • George EF, Sherrington PD (1984) Plant propagation by tissue culture. Eastern Press, England

    Google Scholar 

  • Gomma NH, Pico FX (2011) Seed germination, seedling traits, and bank of the tree Moringa peregrina (Moringaceae) in a hyper-arid environment. Am J Bot 98:1024–1030

    Article  Google Scholar 

  • Goto S, Thakur RC, Ishii K (1998) Determination of genetic stability in long-term micropropagated shoots of Pinus thunbergii Parl. using RAPD markers. Plant Cell Rep 18:193–197

    Article  CAS  Google Scholar 

  • Isabel N, Tremblay L, Michaud M, Tremblay FM, Bousquet J (1993) RAPDs as an aid to evaluate the genetic integrity of somatic embryogenesis-derived populations of Picea mariana (Mill.) B.S.P. Theor Appl Genet 86:81–87

    Article  CAS  Google Scholar 

  • Islam S, Jahan M, Khatun R (2005) In vitro regeneration and multiplication of year-round fruit bearing Moringa oleifera L. J Biol Sci 5:145–148

    Article  Google Scholar 

  • Kant T, Tomar UK, Prajapati S, Parmar AK (2010) in vitro propagation as a viable conservation strategy for Commiphora wightii, an endangered medicinally important desert tree, India. Conserv Evid 7:94–99

    Google Scholar 

  • Kapai V, Priyanka Kapoora P, Usha Raoa UI (2010) In vitro propagation for conservation of rare and threatened plants of India. Int J Biol Technol 1:1–14

    Google Scholar 

  • Khalafalla M, Daffalla H, Abdellatef E, Agabna E, El-Shemy H (2011) Establishment of an in vitro micropropagation protocol for Boscia senegalensis (Pers.) Lam. ex Poir. J Zhejiang Univ Sci B 12:303–312

    Article  PubMed  Google Scholar 

  • Koheil MA, Hussein MA, Othman SM, El-Haddad A (2011) Antiinflammatory and antioxidant activities of Moringa peregrine seeds. Free Radic Antioxid 1:49–61

    Article  CAS  Google Scholar 

  • Lalas S, Gortzi O, Athanasiadis V, Tsaknis J, Chinou I (2012) Determination of antimicrobial activity and resistance to oxidation of Moringa peregrina seed oil. Molecules 17:2330–2334

    Article  PubMed  CAS  Google Scholar 

  • Marfori E (2010) Clonal micropropagation of Moringa oleifera L. Philipp Agric Sci 93:454–457

    Google Scholar 

  • Martins M, Sarmento D, Oliveira MM (2004) Genetic stability of micropropagated almond plantlets as assessed by RAPD and ISSR markers. Plant Cell Rep 23:492–496

    Article  PubMed  CAS  Google Scholar 

  • Mngomba S, Toit E, Akinnifesi F, Venter H (2007) Repeated exposure of jacket plum (Pappea capensis) micro-cuttings to indole-3-butyric acid (IBA) improved in vitro rooting capacity. S Afr J Bot 73:230–235

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:472–497

    Article  Google Scholar 

  • Murray HG, Thompson WF (1980) Rapid isolation of high molecular weight DNA. Nucleic Acids Res 8:4321–4325

    Article  PubMed  CAS  Google Scholar 

  • Nas MN, Bolek Y, Sevgin N (2010) The effects of explant and cytokinin type on regeneration of Prunus microcarpa. Sci Hortic 126:88–94

    Article  CAS  Google Scholar 

  • Prathanturarug S, Rungthipwan P, Thaya J, Wongsatit C, Promchit S (2012) In vitro propagation of Stemona hutanguriana W.Chuakul, an endangered medicinal plant. Physiol Mol Biol Plants 18:281–286

    Article  CAS  Google Scholar 

  • Saini RK, Shetty NP, Giridhar P, Ravishankar GA (2012) Rapid in vitro regeneration method for Moringa oleifera and performance evaluation of field grown nutritionally enriched tissue cultured plants. 3. Biotech 2:187–192

    Google Scholar 

  • Salvi ND, George L, Eapen S (2001) Plant regeneration from leaf base callus of turmeric and random amplified polymorphic DNA analysis of regenerated plants. Plant Cell Tissue Organ Cult 66:113–119

    Article  CAS  Google Scholar 

  • Steinitz B, Tabib Y, Gaba V, Gefen T, Vaknin Y (2009) Vegetative micro-cloning to sustain biodiversity of threatened Moringa species. In vitro Cell Dev Biol Plant 45:65–71

    Article  Google Scholar 

  • Stephenson KK, Fahey JW (2004) Development of tissue culture methods for the rescue and propagation of endangered Moringa spp. germplasm. Econ Bot 58:116–124

    Article  Google Scholar 

  • Tang W (2001) In vitro regeneration of loblolly pine and random amplified polymorphic DNA analyses of regenerated plantlets. Plant Cell Rep 20:163–168

    Article  CAS  Google Scholar 

  • Tiwari KN, Sharma NC, Tiwari V, Singh BD (2000) Micropropagation of Centella asiatica (L.), a valuable medicinal herb. Plant Cell Tissue Organ Cult 63:179–185

    Article  CAS  Google Scholar 

  • Varshney A, Lakshmikumaran M, Srivastava PS, Dhawan V (2001) Establishment of genetic fidelity of in vitro-raised Lilium bulblets through RAPD markers. In vitro Cell Dev Biol Plant 37:227–231

    Article  CAS  Google Scholar 

  • Zaghloul MS, Abd El-Wahab RH, Moustafa AA (2010) Ecological assessment and phenotypic and fitness variation of Sinai’s remnant populations of Moringa peregrina. Appl Ecol Environ Res 8:351–366

    Google Scholar 

Download references

Acknowledgement

The Authors would like to thank Yarmouk University/Jordan for providing the required facilities and the collaborative and interdisciplinary research environment.

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Authors and Affiliations

  1. Department of Biological Sciences, Yarmouk University, Irbid, Jordan

    Wesam Al Khateeb, Eman Bahar, Jamil Lahham & Emad Hussein

  2. Department of Biological Sciences, University of Manitoba, Winnipeg, Canada

    Dana Schroeder

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  1. Wesam Al Khateeb
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  2. Eman Bahar
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  3. Jamil Lahham
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  4. Dana Schroeder
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  5. Emad Hussein
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Correspondence to Wesam Al Khateeb.

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Al Khateeb, W., Bahar, E., Lahham, J. et al. Regeneration and assessment of genetic fidelity of the endangered tree Moringa peregrina (Forsk.) Fiori using Inter Simple Sequence Repeat (ISSR). Physiol Mol Biol Plants 19, 157–164 (2013). https://doi.org/10.1007/s12298-012-0149-z

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  • DOI: https://doi.org/10.1007/s12298-012-0149-z

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