Abstract
The Santalum peroxidase was extracted from the leaves and precipitated with double volume of chilled acetone. The optimum percent relative activity for the Santalum peroxidase was observed at pH 5.0 and 50 °C temperature. The Santalum peroxidase per cent relative activity was stimulated in the presence of phenolic compounds like ferrulic acid and caffeic acids; however, indole-3-acetic acid (IAA) and protocatechuic acid act as inhibitors. All divalent cations Fe2+, Mn2+, Mg2+, Cu2+ and Zn2+ stimulate the relative activity of the Santalum peroxidase at concentration of 2.0 μM. Amino acids like L-alanine and L-valine activate the per cent relative activity, while L-proline and DL-methionine showed moderate inhibition for the Santalum peroxidase. However, a very low a concentration of cysteine acts as a strong inhibitor of Santalum peroxidase at the concentration of 0.4 mM. Native polyacrylamide gel electrophoresis (Native-PAGE) was performed for isoenzyme determination and two bands were observed. Km and Vmax values were calculated from Lineweaver-Burk graph. The apparent Vmax/Km value for O-dianisidine and H2O2 were 400 and 5.0 × 105 Units/min/mL respectively.
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The authors are highly thankful to Department of Biotechnology, D. D. U. University, Gorakhpur, (U. P.) India, for providing necessary financial support to carrying out the research work.
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Kumar, P., Kamle, M. & Singh, J. Biochemical characterization of Santalum album (Chandan) leaf peroxidase. Physiol Mol Biol Plants 17, 153–159 (2011). https://doi.org/10.1007/s12298-011-0054-x
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DOI: https://doi.org/10.1007/s12298-011-0054-x