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Determination of the Roles of Endothelial Nitric Oxide Synthase 4VNTR (4a/b), G894T, T786C Gene Variations in the Bladder Cancer Development

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Abstract

The aim of this study is to determine the roles of eNOS gene variations in BCA development. Our study included 91 patients diagnosed with BCA and 91 healthy controls. eNOS 4VNTR (4a/b), T786C and G894T gene variations genotype distributions were determined by PCR and RFLP methods. The significant difference was determined between these groups in terms of eNOS T786C and eNOS G894T gene variations genotype distributions (p < 0.05). TT genotype for G894T gene variation and CC genotype for T786C gene variation were detected higher in patients. The CC genotype of T786C gene variation was detected significantly higher in male patients than in male controls (p < 0.05). In addition, aa-TT, ab-TT, bb-TT haplotypes of 4VNTR (4a/b)-G894T gene variations, aa-CC, ab-CC, bb-CC haplotypes of 4VNTR (4a/b)-T786C gene variations and TT-TT, TT-CC, TT-CT, GG-CC, GT-CC haplotypes of G894T-T786C gene variations were observed in patient group more than control group. The significant difference was detected between these groups in terms of eNOS (G894T-T786C) haplotypes (p < 0.05). In our study, eNOS T786C and eNOS G894T gene variations were determined important genetic risk factor in the Thrace population of Turkey.

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Acknowledgements

This study was performed in departments of Biophysics and Urology in Faculty of Medicine in Trakya University.

Funding

This study was not supported by any specific grant from funding.

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Authors and Affiliations

  1. Department of Biophysics, Faculty of Medicine, Trakya University, 22030, Edirne, Turkey

    Arzu Ay

  2. Department of Biophysics, Faculty of Medicine, Haliç University, 34060, Istanbul, Turkey

    Nevra Alkanli

  3. Department of Urology, Faculty of Medicine, Trakya University, 22030, Edirne, Turkey

    Gokhan Cevik

Authors
  1. Arzu Ay
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  2. Nevra Alkanli
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  3. Gokhan Cevik
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by [NA], [AA], and [GC]. All authors approved the final manuscript. The corresponding author attests that all listed authors meet the authorship criteria and that no other authors meeting the criteria have been omitted.

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Correspondence to Arzu Ay or Nevra Alkanli.

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The authors declare that they have no conflict of interest.

Ethical approval

For our study, ethics committee approval was obtained with the TÜTF-BAEK 2020/240 protocol code from Trakya University Faculty of Medicine Non-Invasive Clinical Research Ethics Committee.

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Signed informed consent form was collected from each individual in the patient group with BCA and healthy control group.

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Ay, A., Alkanli, N. & Cevik, G. Determination of the Roles of Endothelial Nitric Oxide Synthase 4VNTR (4a/b), G894T, T786C Gene Variations in the Bladder Cancer Development. Ind J Clin Biochem 39, 92–100 (2024). https://doi.org/10.1007/s12291-022-01090-4

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  • DOI: https://doi.org/10.1007/s12291-022-01090-4

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