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Estimation of 24 h Urine Protein Versus Spot Urine Protein Creatinine Ratio in Patients with Kidney Disease

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Abstract

This cross-sectional study was undertaken to compare 24-h urine protein estimation with spot urine protein-creatinine ratio (PCR). 72 patients having proteinuria of > 150 mg/day were included in this study. 24-h urine total protein (UTP) test were also asked to give their mid-morning spot urine sample the following day for urine protein-creatinine ratio. The subjects were grouped according to their UTP into Groups as described follows: Group A < 1 g/day, Group B 1–3.5 g/day and Group C > 3.5 g/day. Pearson’s correlation (r) and paired Student’s T test were done. The paired T test showed no difference in the UTP and PCR in the subgroup having qualitative protein of zero and in group A. There was no agreement between the two methods used for quantitation. In the overtly proteinuric patients, the correlation between the UTP and PCR was positive in group A which was statistically significant (p = 0.05) whereas it was not significant (p = 0.07) in group B. Though there was a negative correlation in the massive proteinuria group C it was not significant (p = 0.65). Our study demonstrates that spot PCR can be reliable parameter for initial diagnostic criterion of proteinuria but for follow-up measurements and for proteinuria > 0.5 gm/day, 24-h UTP is the single most accurate measurement of protein excretion.

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Availability of data and material

The deidentified data is published in Mendeley Data, and is cited as Sahu, Suchanda (2020), “24 h urine protein vs UPCR”, Mendeley Data, V1, https://doi.org/10.17632/4c9z3n29pw.1

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Acknowledgements

This study was non-funded project. We acknowledge the support of our study participants for their consent and cooperation.

Funding

No funding was received for conducting this study.

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Authors and Affiliations

  1. Department of Biochemistry, All India Institute of Medical Sciences, PO Dumduma, Bhubaneswar, 751019, Odisha, India

    Suchanda Sahu

  2. Department of Pediatrics, All India Institute of Medical Sciences, Bhubaneswar, Odisha, India

    Joseph John

  3. Department of Biochemistry, Shree Narayana Institute of Medical Sciences, Ernakulam, Kerala, India

    Asha Augusty

Authors
  1. Suchanda Sahu
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  2. Joseph John
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  3. Asha Augusty
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Contributions

The authors SS, JJ, AA conceptualized the project, carried out the experiment and approved the final manuscript. SS did the data analysis and prepared the draft manuscript.

Corresponding author

Correspondence to Suchanda Sahu.

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Conflict of interest

The authors declare that they have no competing interest in the study conducted.

Ethical approval

Ethical approval was waived by the local Ethics Committee of Sree Narayana Medical College, Cochin in view of the retrospective nature of the study and all the procedures being performed were part of the routine care.

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Sahu, S., John, J. & Augusty, A. Estimation of 24 h Urine Protein Versus Spot Urine Protein Creatinine Ratio in Patients with Kidney Disease. Ind J Clin Biochem 37, 361–364 (2022). https://doi.org/10.1007/s12291-020-00943-0

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  • DOI: https://doi.org/10.1007/s12291-020-00943-0

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