Skip to main content
SpringerLink
Log in

Effect of Nitric Oxide Donor on Metabolism of Apheresis Platelets

  • Original Article
  • Published:
Indian Journal of Hematology and Blood Transfusion Aims and scope Submit manuscript

Abstract

The aim of this study is to investigate the effects of a nitric oxide (NO) donor S-nitrosoglutathione (GSNO) on the metabolism and improvement of preservation quality in apheresis platelets. A GSNO solution with a certain concentration was added into fresh apheresis platelets, and the parameters associated with platelet morphology, metabolism and function were temporally monitored for 7 days. The results showed that the NO level in GSNO group were remarkably higher than those in the control group during the whole storage stage. No significant morphology or function difference was observed between the control and GSNO groups such as Platelet count, platelet distribution width, mean platelet volume and mitochondrial metabolic activity. But in metabolism there are something differences from morphology data: pCO2, pO2, cHCO3 − were also found to have no clear difference between the control and GSNO groups; the lactic acid content, sugar consumption and Lactate dehydrogenase activity in the GSNO group were lower than that in the control group at some time point; and pH values in the GSNO group were higher than the control group. Our study discovered that the NO donor GSNO can reduce the metabolism and maintain the cellular characteristics of platelets in vitro during the platelet storage period.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Devine DV, Serrano K (2010) The platelet storage lesion. Clin Lab Med 30(2):475–487

    Article  PubMed  Google Scholar 

  2. Thon JN, Schubert P, Devine DV (2008) Platelet storage lesion: a new understanding from a proteomic perspective. Transfus Med Rev 22(4):268–279

    Article  PubMed  Google Scholar 

  3. Shrivastava M (2009) The platelet storage lesion. Transfus Apher Sci 41(2):105–113

    Article  PubMed  Google Scholar 

  4. Zhang JG, Carter CJ, Culibrk B, Devine DV, Levin E, Scammell K, Weiss S, Gyongyossy-Issa MI (2008) Buffy-coat platelet variables and metabolism during storage in additive solutions or plasma. Transfusion 48(5):847–856

    Article  PubMed  CAS  Google Scholar 

  5. Li J, Xia Y, Bertino AM, Coburn JP, Kuter DJ (2000) The mechanism of apoptosis in human platelets during storage. Transfusion 40(11):1320–1329

    Article  PubMed  CAS  Google Scholar 

  6. Solheim BG, Flesland O, Seghatchian J, Brosstad F (2004) Clinical implications of red blood cell and platelet storage lesions: an overview. Transfus Apher Sci 31(3):185–189

    Article  PubMed  CAS  Google Scholar 

  7. Tulika C, Ashish G, Ashutosh K, Sheeba A (2011) Morphological and functional changes in random donor platelets stored for seven days in platelet additive solution. Int J Blood Transfus Immunohematol 1:20

    Article  Google Scholar 

  8. van der Meer PF, de Korte D (2011) Platelet preservation: agitation and containers. Transfus Apher Sci 44(3):297–304

    Article  PubMed  Google Scholar 

  9. Sandgren P, Saeed K (2011) Storage of buffy-coat-derived platelets in additive solution: in vitro effects on platelets of the air bubbles and foam included in the final unit. Blood Transfus 9(2):182–188

    PubMed  PubMed Central  Google Scholar 

  10. Schubert P, Devine DV (2010) Towards targeting platelet storage lesion-related signaling pathways. Blood Transfus 8(Suppl 3):s69–s72

    PubMed  PubMed Central  Google Scholar 

  11. Dumont LJ, AuBuchon JP, Gulliksson H, Slichter SJ, Elfath MD, Holme S, Murphy JR, Rose LE, Popovsky MA, Murphy S (2006) In vitro pH effects on in vivo recovery and survival of platelets: an analysis by the BEST collaborative. Transfusion 46(8):1300–1305

    Article  PubMed  Google Scholar 

  12. Shapira S, Friedman Z, Shapiro H, Presseizen K, Radnay J, Ellis MH (2000) The effect of storage on the expression of platelet membrane phosphatidylserine and the subsequent impacton the coagulant function of stored platelets. Transfusion 40(10):1257–1263

    Article  PubMed  CAS  Google Scholar 

  13. Seghatchian J, Krailadsiri P (2001) Platelet storage lesion and apoptosis: are they related? Transfus Apher Sci 24(1):103–105

    Article  PubMed  CAS  Google Scholar 

  14. Wood EM, Colton E, Yomtovian RA, Currie LM, Connor J, Anderson JM (2000) Prevention of monocyte adhesion and inflammatory cytokine production during blood platelet storage: an in vitro model with implications for transfusion practice. J Biomed Mater Res 51(2):147–154

    Article  PubMed  CAS  Google Scholar 

  15. Radomski MW, Zakar T, Salas E (1996) Nitric oxide in platelets. Methods Enzymol 269:88–107

    Article  PubMed  CAS  Google Scholar 

  16. Sener A, Egemen G, Cevik O, Yanikkaya-Demirel G, Apikoglu-Rabus S, Ozsavci D (2013) In vitro effects of nitric oxide donors on apoptosis and oxidative/nitrative protein modifications in ADP-activated platelets. Hum Exp Toxicol 32(3):225–235

    Article  PubMed  CAS  Google Scholar 

  17. Riss TL, Moravec RA (1992) Comparison of MTT, XTT, and a novel tetrazolium compound MTS for in vitro proliferation and chemosensitivity assays. Mol Biol Cell 3:184–194

    Google Scholar 

  18. Xiong J, Zhu J, Dai W, Dong C, Qiu Q, Li C (2017) Integrating gut microbiota immaturity and disease-discriminatory taxa to diagnose the initiation and severity of shrimp disease. Environ Microbiol 19(4):1490–1501

    Article  PubMed  Google Scholar 

  19. Dai W, Zhang J, Tu Q, Deng Y, Qiu Q, Xiong J (2017) Bacterioplankton assembly and interspecies interaction indicating increasing coastal eutrophication. Chemosphere 177:317–325

    Article  PubMed  CAS  Google Scholar 

  20. Xiong J, Zhu J, Wang K, Wang X, Ye X, Liu L, Zhao Q, Hou M, Qiuqian L, Zhang D (2014) The temporal scaling of bacterioplankton composition: high turnover and predictability during shrimp cultivation. Microb Ecol 67(2):256–264

    Article  PubMed  Google Scholar 

  21. Cesar J, DiMinno G, Alam I, Silver M, Murphy S (1987) Plasma free fatty acid metabolism during storage of platelet concentrates for transfusion. Transfusion 27(5):434–437

    Article  PubMed  CAS  Google Scholar 

  22. Gulliksson H (2003) Defining the optimal storage conditions for the long-term storage of platelets. Transfus Med Rev 17(3):209–215

    Article  PubMed  Google Scholar 

  23. Gkaliagkousi E, Ritter J, Ferro A (2007) Platelet-derived nitric oxide signaling and regulation. Circ Res 101(7):654–662

    Article  PubMed  CAS  Google Scholar 

  24. Marjanovic JA, Stojanovic A, Brovkovych VM, Skidgel RA, Du X (2008) Signaling-mediated functional activation of inducible nitric-oxide synthase and its role in stimulating platelet activation. J Biol Chem 283(43):28827–28834

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  25. Oberprieler NG, Roberts W, Riba R, Graham AM, Homer-Vanniasinkam S, Naseem KM (2007) cGMP-independent inhibition of integrin alphaIIbbeta3-mediated platelet adhesion and outside-in signalling by nitric oxide. FEBS Lett 581(7):1529–1534

    Article  PubMed  CAS  Google Scholar 

  26. Shiri R, Yari F, Ahmadinejad M, Vaeli S, Tabatabaei MR (2014) The caspase-3 inhibitor (peptide Z-DEVD-FMK) affects the survival and function of platelets in platelet concentrate during storage. Blood research 49(1):49–53

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  27. Picker SM, Schneider V, Oustianskaia L, Gathof BS (2009) Cell viability during platelet storage in correlation to cellular metabolism after different pathogen reduction technologies. Transfusion 49(11):2311–2318

    Article  PubMed  CAS  Google Scholar 

  28. Tynngard N, Wallstedt M, Sodergren AL, Faxalv L, Ramstrom S (2015) Platelet adhesion changes during storage studied with a novel method using flow cytometry and protein-coated beads. Platelets 26(2):177–185

    Article  PubMed  CAS  Google Scholar 

  29. Deyhim MR, Mesbah-Namin SA, Yari F, Taghikhani M, Amirizadeh N (2015) L-carnitine effectively improves the metabolism and quality of platelet concentrates during storage. Ann Hematol 94(4):671–680

    Article  PubMed  CAS  Google Scholar 

  30. Tynngard N (2009) Preparation, storage and quality control of platelet concentrates. Transfus Apher Sci 41(2):97–104

    Article  PubMed  Google Scholar 

  31. Crane MS, Ollosson R, Moore KP, Rossi AG, Megson IL (2002) Novel role for low molecular weight plasma thiols in nitric oxide-mediated control of platelet function. J Biol Chem 277(49):46858–46863

    Article  PubMed  CAS  Google Scholar 

  32. Skrzypchak AM, Lafayette NG, Bartlett RH, Zhou Z, Frost MC, Meyerhoff ME, Reynolds MM, Annich GM (2007) Effect of varying nitric oxide release to prevent platelet consumption and preserve platelet function in an in vivo model of extracorporeal circulation. Perfusion 22(3):193–200

    Article  PubMed  Google Scholar 

  33. Wu T, Zhang C, Wang Z, Liu J, Li H, Zhou W, Wang S (2013) Inhibitory role of S-nitrosoglutathione in the aggregation of frozen platelets, and its effect on the expression of membrane glycoproteins. Exp Ther Med 6(3):831–839

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  34. Lee JH, Kim JT, Cho YG (2008) Effect of nitric oxide on the cryopreservation of platelets. Korean J Lab Med 28(2):136–143

    Article  PubMed  CAS  Google Scholar 

  35. Wong K, Li X (2004) Nitric oxide infusion alleviates cellular activation during preparation, leukofiltration and storage of platelets. Transfus Apher Sci 30(1):29–39

    Article  PubMed  Google Scholar 

Download references

Acknowledgements

We thank Yan Wang and Huihui Feng for contribution to serve the blood donors and PCs collection. This work was supported by the Grants from the Project of Science and Technology Department of Ningbo (No. 2017C50046) and Project of Health Department of Ningbo (No. 2016A17).

Author information

Author notes

  1. Lu Yu and Shifang Yu have contributed equally to this work.

Authors and Affiliations

  1. The Ningbo Central Blood Station, Ningbo, China

    Lu Yu, Yunlei He, Deyi Xu, Kai Huang & Gang Deng

  2. The Department of Transfusion Medicine, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, China

    Shifang Yu

  3. The Department of Cardiology, The Fourth Affiliated Hospital of Zhejiang University School of Medicine, Yiwu, China

    Qiming Li

  4. The Department of Laboratory Medicine, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, China

    Qiang Li

Authors
  1. Lu Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shifang Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yunlei He
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Qiming Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Deyi Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Kai Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Gang Deng
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Qiang Li
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Author’s Contributions

QL and GD conceived and designed the experiments; LY, SY, YH and GD performed the experiments and evaluated the results; QL, QL, DX and KH contributed reagents and materials; GD, LY and SY wrote the manuscript.

Corresponding author

Correspondence to Qiang Li.

Ethics declarations

Conflict of interest

None of the authors have any conflict of interest to declare.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yu, L., Yu, S., He, Y. et al. Effect of Nitric Oxide Donor on Metabolism of Apheresis Platelets. Indian J Hematol Blood Transfus 34, 517–523 (2018). https://doi.org/10.1007/s12288-017-0881-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12288-017-0881-1

Keywords

Search

Navigation