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Establishment of a standardized gene-expression analysis system using formalin-fixed, paraffin-embedded, breast cancer specimens

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Abstract

Background

It has recently being emphasized that gene-expression profiles are important clinical decision-making tools, and as such must be standardized across hospital laboratories in the same way as pathological investigations. In this study our objective was to independently establish a standardized gene-expression assay system using routinely processed, formalin-fixed, paraffin-embedded (FFPE) tissues.

Methods

To verify gene expression by quantitative real-time polymerase chain reaction, the most stably expressed reference genes were explored using 30 matched FFPE and fresh frozen (FF) tissues. FFPE specimens from 290 female breast cancer patients were used for further RNA extraction; ESR1 and PGR were measured using 203 matched FFPE and FF specimens and normalized to these reference genes.

Results

RNA extracted from FFPE specimens was highly degraded, but almost the same selection of genes was identified—TAF, PUM1, and ACTB, and, for FFPE specimens only, FKBP15. Eventually 88.6% of all the FFPE samples were identified as quantitatively and qualitatively adequate for downstream analysis. The results revealed good correlation and excellent concordance with ERα and PgR protein expression evaluated by immunohistochemistry. Moreover, the distribution of ESR1 and PGR gene expression values was quite reasonable, reflecting differences between the transcriptional mechanisms of the respective genes.

Conclusions

We successfully confirmed that our gene-expression analysis system provides good quality control for larger scale assays; it may therefore be suitable for development, in the near future, of a multiple gene assay as a routine clinical judgment tool.

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Acknowledgments

We thank Y. Azakami for excellent technical support and A. Okabe, for excellent clinical data management.

Conflict of interest

The authors declare that they have no conflict of interest.

Author information

Authors and Affiliations

  1. Department of Breast and Endocrine Surgery, Graduate School of Medical Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto, Kumamoto, 860-8556, Japan

    Mutsuko Ibusuki, Peifen Fu, Satoko Yamamoto, Saori Fujiwara, Yutaka Yamamoto & Hirotaka Iwase

  2. Department of General Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Road, Hangzhou, 310003, People’s Republic of China

    Peifen Fu

  3. Department of Surgical Pathology, Graduate School of Medical Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto, Kumamoto, 860-8556, Japan

    Yumi Honda & Ken-ichi Iyama

Authors
  1. Mutsuko Ibusuki
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  2. Peifen Fu
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  3. Satoko Yamamoto
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  4. Saori Fujiwara
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  5. Yutaka Yamamoto
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  7. Ken-ichi Iyama
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  8. Hirotaka Iwase
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Correspondence to Mutsuko Ibusuki.

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Ibusuki, M., Fu, P., Yamamoto, S. et al. Establishment of a standardized gene-expression analysis system using formalin-fixed, paraffin-embedded, breast cancer specimens. Breast Cancer 20, 159–166 (2013). https://doi.org/10.1007/s12282-011-0318-x

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  • DOI: https://doi.org/10.1007/s12282-011-0318-x

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