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The role of Jacalin-related lectin gene AOL_s00083g511 in the development and pathogenicity of the nematophagous fungus Arthrobotrys oligospora

  • Microbial Genetics, Genomics and Molecular Biology
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Abstract

Arthrobotrys oligospora is a model species of nematophagous fungi and has great potential for the biological control of nematode diseases. Lectin is a protein that binds to carbohydrates and their complexes with high specificity, which mediates recognition events in various physiological and pathological processes. This study aimed to investigate the role of the Jacalin-related lectin (JRL) gene, AOL_s00083g511, in A. oligospora development. Through a homology recombination approach, we obtained the AOL_s00083g511 knockout mutant strain (Ag511). Next, the biological characteristics of the Ag511 mutant strain, including growth rate, conidia germination rate, adaptation to environmental stresses, and nematocidal activity, were compared with those of the wild-type (WT) strain. The results showed that the JRL gene AOL_s00083g511 did not affect fungal growth, conidia germination, 3D-trap formation, and the ability of A. oligospora to prey on nematodes significantly. We speculate that this phenomenon may be caused by a loss of the key β1-β2 loops in the AOL_ s00083g511-encoded JRL domain and an intrinsic genetic compensation of AOL_s00083g511 in this fungus. The growth rates of both strains on high salt or surfactant media were similar; however, in the strong oxidation medium, the growth rate of the Ag511 mutant was significantly lower than that of the WT strain, indicating that AOL_s00083g511 might play a role in oxidative stress resistance. These findings provide a basis for further analysis of the related functions of the JRL gene in A. oligospora and their potential roles in the biological control of nematodes in the future.

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Acknowledgements

This study was supported, at least in part, by the Natural Science Foundation of China (Y.W., grant Nos. 31770066 and 31470218), the Open Fund for Discipline Construction, Institute of Physical Science and Information Technology of Anhui University (Y.W.), and the Outstanding Talents Program of Anhui University, China (Y.W.).

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Authors and Affiliations

  1. School of Life Sciences, Anhui University, Hefei, 230601, P. R. China

    Xinyuan Dong, Jiali Si, Guanghui Zhang, Zhen Shen, Li Zhang, Kangliang Sheng, Jingmin Wang, Xiaowei Kong, Xiangdong Zha & Yongzhong Wang

  2. Key Laboratory of Human Microenvironment and Precision Medicine of Anhui Higher Education Institutes, Anhui University, Hefei, 230601, P. R. China

    Xinyuan Dong, Jiali Si, Guanghui Zhang, Zhen Shen, Li Zhang, Kangliang Sheng, Jingmin Wang, Xiaowei Kong, Xiangdong Zha & Yongzhong Wang

  3. Anhui Key Laboratory of Modern Biomanufacturing, Hefei, 230601, P. R. China

    Xinyuan Dong, Jiali Si, Guanghui Zhang, Zhen Shen, Li Zhang, Kangliang Sheng, Jingmin Wang, Xiaowei Kong, Xiangdong Zha & Yongzhong Wang

  4. Institute of Physical Science and Information Technology, Anhui University, Hefei, 230601, P. R. China

    Yongzhong Wang

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  1. Xinyuan Dong
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  2. Jiali Si
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  3. Guanghui Zhang
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  5. Li Zhang
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Correspondence to Yongzhong Wang.

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Dong, X., Si, J., Zhang, G. et al. The role of Jacalin-related lectin gene AOL_s00083g511 in the development and pathogenicity of the nematophagous fungus Arthrobotrys oligospora. J Microbiol. 59, 736–745 (2021). https://doi.org/10.1007/s12275-021-1029-4

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  • DOI: https://doi.org/10.1007/s12275-021-1029-4

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