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Functionalized CVD monolayer graphene for label-free impedimetric biosensing

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Abstract

Recent advances in large area graphene growth have led to many applications in different areas. In the present study, chemical vapor deposited (CVD) monolayer graphene supported on glass substrate was examined as electrode material for electrochemical biosensing applications. We report a facile strategy for covalent functionalization of CVD monolayer graphene by electrochemical reduction of carboxyphenyl diazonium salt prepared in situ in acidic aqueous solution. The carboxyphenyl-modified graphene is characterized using Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), and atomic force microscopy (AFM), as well as electrochemical impedance spectroscopy (EIS). We also show that the number of grafted carboxyphenyl groups on the graphene surface can be controlled by the number of cyclic voltammetry (CV) scans used for electrografting. We further present the fabrication and characterization of an immunosensor based on immobilization of ovalbumin antibody on the graphene surface after the activation of the grafted carboxylic groups via EDC/NHS chemistry. The binding between the surface-immobilized antibodies and ovalbumin was then monitored using Faradaic EIS in [Fe(CN)6]3−/4− solution. The percentage change of charge transfer resistance (R ct) after binding exhibited a linear dependence for ovalbumin concentrations ranging from 1.0 pg·mL−1 to 100 ng·mL−1, with a detection limit of 0.9 pg·mL−1. Our results indicate good sensitivity of the developed functionalized CVD graphene platform, paving the way for using CVD monolayer graphene in a variety of electrochemical biosensing devices.

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Authors and Affiliations

  1. Institut national de la recherche scientifique, Centre — Energie, Matériaux et Télécommunications, 1650, Boul. Lionel Boulet, Varennes, Québec, J3X 1S2, Canada

    Shimaa Eissa & Chaker Tlili

  2. Département de Chimie et Biochimie, Université du Québec à Montréal, Montréal, Québec, H3C 3P8, Canada

    Shimaa Eissa, Gaston Contreras Jimenez, Farzaneh Mahvash, Abdeladim Guermoune & Mohamed Siaj

  3. Dept. of Electrical and Computer Engineering, McGill University, Montréal, Québec, H3A 2A7, Canada

    Farzaneh Mahvash & Thomas Szkopek

  4. Cranfield Health, Vincent Building, Cranfield University, Cranfield, Bedfordshire, UK

    Mohammed Zourob

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  1. Shimaa Eissa
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  2. Gaston Contreras Jimenez
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  3. Farzaneh Mahvash
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  4. Abdeladim Guermoune
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  5. Chaker Tlili
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  8. Mohamed Siaj
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Correspondence to Mohamed Siaj.

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Eissa, S., Jimenez, G.C., Mahvash, F. et al. Functionalized CVD monolayer graphene for label-free impedimetric biosensing. Nano Res. 8, 1698–1709 (2015). https://doi.org/10.1007/s12274-014-0671-0

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  • DOI: https://doi.org/10.1007/s12274-014-0671-0

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