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Chemical constituents of Triticum aestivum and their effects on adipogenic differentiation of 3T3-L1 preadipocytes

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Abstract

In this report, we investigated the anti-obesity effect of wheat sprouts and their component compounds. Twenty compounds (1–20) were isolated from Triticum aestivum. Among them, glycolipids 1–5 were determined for the first time from T. aestivum and its sprouts. The HPLC analysis demonstrated that compounds 1–3, 5, 8, 12, and 14 were major peak in the HPLC chromatogram of the active fraction. The effects of the compounds on lipid accumulation were assessed at concentrations ranging from 1.0 to 100 μM. At concentration of 10.0 μM, compounds 1–7, 10−15, and 17–19 significantly decreased lipid accumulation in 3T3-L1 preadipocytes. Glycolipids 1, 2, and phenolic 17 significantly reduced lipid accumulation in the differentiated adipocytes in a concentration-dependent manner. Quantitative analysis based on measurement of the optical density of Oil Red O indicated that, at 100 μM, compounds 1, 2, and 17 reduced lipid accumulation by 41, 37, and 48 %, respectively, compared with the positive control.

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Acknowledgments

This research was financially supported by the Ministry of Knowledge Economy (MKE), Korea Institute for Advancement of Technology (KIAT) through the Inter-ER Cooperation Projects (R0002019) and the Priority Research Center Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology (2009-0093815), Republic of Korea.

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Authors and Affiliations

  1. College of Pharmacy, Chungnam National University, Daejeon, 305-764, Republic of Korea

    Bui Thi Thuy Luyen, Nguyen Phuong Thao, Bui Huu Tai & Young Ho Kim

  2. Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Caugiay, Hanoi, Vietnam

    Nguyen Phuong Thao & Bui Huu Tai

  3. Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University and Wonkwang Oriental Medicines Research Institute, Iksan, Jonbuk, 570-749, Republic of Korea

    Ji Young Lim & Young Mi Lee

  4. Department of Immunolgy and Institute of Medical Sciences, Medical School, Chonbuk National University, Jeonju, Jeonbuk, 561-756, Republic of Korea

    Hyeon Hui Ki & Dae Ki Kim

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  1. Bui Thi Thuy Luyen
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Correspondence to Young Mi Lee or Young Ho Kim.

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Luyen, B.T.T., Thao, N.P., Tai, B.H. et al. Chemical constituents of Triticum aestivum and their effects on adipogenic differentiation of 3T3-L1 preadipocytes. Arch. Pharm. Res. 38, 1011–1018 (2015). https://doi.org/10.1007/s12272-014-0478-2

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  • DOI: https://doi.org/10.1007/s12272-014-0478-2

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