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Hepatoprotective effects of reynosin against thioacetamide-induced apoptosis in primary hepatocytes and mouse liver

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Abstract

The aim of this study was to identify the hepatoprotective effects of reynosin, sesquiterpenes from the leaves of Laurus nobilis, against thioacetamide (TAA)-induced apoptosis in primary hepatocyte cultures and an in vivo mouse model. Rat hepatocytes were isolated and pretreated with 0.13, 0.64, or 3.22 μM reynosin and then exposed to 100 mM TAA. Reynosin treatment significantly inhibited TAA-induced apoptosis and hepatocellular DNA damage in primary rat hepatocytes. We observed an increase in levels of antiapoptotic Bcl-2, Bcl-XL mRNA and a decrease in levels of proapoptotic Bax mRNA following reynosin treatment of hepatocytes. Apoptosis in BALB/c mice was induced with intra-peritoneal injection of 200 mg/kg TAA for 2 weeks every other day. Then reynosin (5 mg/kg) and TAA were intragastrically given for 3 weeks every other day. Aspartate aminotransferase and alanine aminotransferase levels in the blood of mice were decreased in the reynosin administration group. Bcl-2 and Bcl-XL mRNA levels were increased, and the Bax mRNA level was decreased in reynosin-treated mice. Thus, reynosin inhibited TAA-induced apoptosis in primary hepatocytes and an in vivo mouse model.

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Acknowledgments

This research was supported by the Basic Science Research Program of the National Research Foundation of Korea, funded by the Ministry of Education, Science and Technology (grant number 120120392).

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Authors and Affiliations

  1. Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul, 151-742, South Korea

    Soohyun Lim & Woongchon Mar

  2. Department of Animal Biotechnology, Kangwon National University, Chuncheon, 200-701, South Korea

    Sung-Jin Lee

  3. SCH-BioPharm Human Resources, Development Center, Soon Chun Hyang University, Asan, 336-745, South Korea

    Kung-Woo Nam

  4. College of Pharmacy, Kangwon National University, Chuncheon, 200-701, South Korea

    Kyeong Ho Kim

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  1. Soohyun Lim
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  2. Sung-Jin Lee
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  3. Kung-Woo Nam
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  5. Woongchon Mar
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Correspondence to Woongchon Mar.

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Soohyun Lim and Sung-Jin Lee contributed equally to this study.

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Lim, S., Lee, SJ., Nam, KW. et al. Hepatoprotective effects of reynosin against thioacetamide-induced apoptosis in primary hepatocytes and mouse liver. Arch. Pharm. Res. 36, 485–494 (2013). https://doi.org/10.1007/s12272-013-0039-0

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