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Effect of resuscitation after selective cerebral ultraprofound hypothermia on expressions of nerve growth factor and glial cell line-derived neurotrophic factor in the brain of monkey

选择性超深低温断血流复苏促进猴脑中神经生长因子和胶质细胞源性神经营养因子的表达

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Abstract

Objective

To investigate the expression of nerve growth factor (NGF) and glial cell line-derived neurotrophic factor (GDNF) in monkeys of resuscitation after selective cerebral ultraprofound hypothermia and blood flow occlusion.

Methods

The monkeys were immediately removed brain after death in operation of group A (identical temperature perfusion group) and group B (ultraprofound hypothermia perfusion group). Immunohistochemical technique was used to determine frontal cellular expression of NGF and GDNF. Statistics were analyzed by ANOVA analyses with significance level at P < 0.05.

Results

The expressions of NGF and GDNF in the group B were significantly higher than those in the group A (P < 0.05).

Conclusion

NGF and GDNF increased significantly in the monkeys of resuscitation after selective cerebral ultraprofound hypothermia and blood flow occlusion. It may be a protective mechanism for neuron survival and neural function recovery.

摘要

目的

观察常温缺血10 min后选择性超深低温断血流复苏后猴脑中神经生长因子(nerve growth factor, NGF)和胶质细胞源性神经营养因子(glial cell line-derived neurotrophicfactor, GDNF)表达的变化。

方法

等温组及超深低温组实验猴于灌注或复苏死亡后立即开颅取脑, 用NGF和GDNF抗体进行免疫组化染色;对额叶恒定视野内NGF和GDNF 的阳性细胞记数求阳性率, 并统计学分析。

结果

等温组2 只实验猴额叶NGF 和GDNF 有微量表达, 超深低温组4 只实验猴额叶NGF 和GDNF 表达明显上调, 与等温组比较差异均极显著(P < 0.01)。

结论

猴脑选择性超深低温断血流复苏实验可引起NGF和GDNF表达上调, 这可能是防止脑缺血的重要保护机制之一。

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Authors and Affiliations

  1. Department of Neurosurgery, Lishui Central Hospital, Lishui, 323000, China

    Xue-Cai Huang

  2. Department of Neurosurgery, the Second Affiliated Hospital of Kunming Medical College, Kunming, 650101, China

    Wei Xu

  3. Department of Neurosurgery, Renji Hospital, Shanghai Jiaotong University, Shanghai, 200001, China

    Ji-Yao Jiang

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  1. Xue-Cai Huang
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  2. Wei Xu
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Correspondence to Wei Xu.

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Huang, XC., Xu, W. & Jiang, JY. Effect of resuscitation after selective cerebral ultraprofound hypothermia on expressions of nerve growth factor and glial cell line-derived neurotrophic factor in the brain of monkey. Neurosci. Bull. 24, 150–154 (2008). https://doi.org/10.1007/s12264-008-1702-1

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  • DOI: https://doi.org/10.1007/s12264-008-1702-1

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