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Enhancement of milk protein expression in mammary epithelial cells via co-culturing with preadipocyte cells

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Abstract

Mammary gland development is critically dependent on the interactions between its stromal and epithelial compartments. In this study, we established a co-culture of bovine mammary epithelial Mac-T cells and murine preadipocyte 3T3-L1 cells. Mac-T cells were co-cultured with 3T3-L1 cells for four days and production of milk proteins was induced for three days. After seven days of co-culturing, the number of alveolar-like colonies in the presence of 3T3-L1 cells was significantly higher than that in control group. Expression levels of αS1-casein and β-casein mRNAs were significantlyincreased by co-culturing with 3T3-L1 cells. In addition, casein protein production was significantly higher in the co-culture of Mac-T cells with 3T3-L1 cells than in control Mac-T cells. Substances that induced casein production in Mac-T cells also stimulated adipogenesis in 3T3-L1 cells. We suggest that a co-culture system of bovine mammary epithelial cells and preadipocyte cells is an efficient method for in vitro milk protein production.

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Author notes

  1. These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Beef and Dairy Science, Korea National College of Agricultures and Fisheries, Jeonju, 54874, Korea

    Won-Young Lee & Joon Mo Yeo

  2. Department of Stem Cell and Regenerative Biology, Konkuk University, Seoul, 05029, Korea

    Hyun-Jung Park & Hyuk Song

  3. Dairy Science Division, National Institute of Animal Science, RDA, Cheonan, 31000, Korea

    Ha Yeon Jeong

Authors
  1. Won-Young Lee
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  2. Hyun-Jung Park
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  3. Joon Mo Yeo
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  4. Ha Yeon Jeong
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  5. Hyuk Song
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Correspondence to Hyuk Song.

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Lee, WY., Park, HJ., Yeo, J.M. et al. Enhancement of milk protein expression in mammary epithelial cells via co-culturing with preadipocyte cells. Biotechnol Bioproc E 22, 556–560 (2017). https://doi.org/10.1007/s12257-017-0264-3

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  • DOI: https://doi.org/10.1007/s12257-017-0264-3

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