Abstract
Porcine reproductive and respiratory syndrome is caused by the PRRS virus (PRRSV), which has six structural proteins (GP2, GP3, GP4, GP5, M and N). GP5 and N protein are important targets for serological detection by enzyme-linked immunosorbent assay (ELISA) and other methods. Toward this goal, we developed an indirect ELISA with recombinant GP5 antigens and this method was validated by comparison to the LSI PRRSV-Ab ELISA kit. The results indicated that the optimal concentration of coated recombinant antigen was 0.2 μg/well for a serum dilution of 1:40. The rate of agreement with the LSI PRRSV-Ab kit was 88.7% (266/300). These results support the potential use of recombinant GP5 as an antigen for indirect ELISA to detect PRRSV antibodies in pigs.
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Foundation item: Specific public service sectors of agriculture research (200803020).
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Chen, Y., Tian, H., He, JH. et al. Indirect ELISA with recombinant GP5 for detecting antibodies to porcine reproductive and respiratory syndrome virus. Virol. Sin. 26, 61–66 (2011). https://doi.org/10.1007/s12250-011-3154-9
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DOI: https://doi.org/10.1007/s12250-011-3154-9