Abstract
To better understand the production of enzymes of industrial interest from microorganisms with biotechnological potential using lignocellulosic biomass, we evaluated the production of endoglucanase and xylanase from Aspergillus tamarii. CAZymes domains were evaluated in the genome, and a screening of the enzymatic potential of A. tamarii in various agricultural biomasses was done. The enzymatic profile could be associated with the biomass complexity, with increased biomass recalcitrance yielding higher activity. A time-course profile defined 48 h of cultivation as the best period for cultivating A. tamarii in sugarcane bagasse reached 12.05 IU/mg for endoglucanase and 74.86 IU/mg for xylanase. Using 0.1% (w/v) tryptone as the only nitrogen source and 12 µmol/L CuSO4 addition had an overall positive effect on the enzymatic activity and protein production. A 22 factorial central composite design was used then to investigate the simultaneous influence of tryptone and CuSO4 on enzyme activity. Tryptone strongly affected enzymatic activity, decreasing endoglucanase activity but increasing xylanase activity. CuSO4 supplementation was advantageous for endoglucanases, increasing their activity, and it had a negative effect on xylanases. But overall, the experimental design increased the enzymatic activity of all biomasses used. For the clean cotton residue, the experimental design was able to reach the highest enzyme activity for endoglucanase and xylanase, with 1.195 IU/mL and 6.353 IU/mL, respectively. More experimental studies are required to investigate how the biomass induction effect impacts enzyme production.
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Abbreviations
- CAZy:
-
Carbohydrate-active enzymes database
- FP :
-
Filter powder
- CC:
-
Clean cotton residue
- DC:
-
Dirty cotton residue
- DP:
-
Dark pulp
- WP:
-
White pulp
- SB:
-
Sugarcane bagasse
- CCD:
-
Central Composite Design
- CCRD:
-
Central Composite Rotatable Design
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Acknowledgements
The authors would like to acknowledge the Brazilian National Council for Scientific and Technological Development (CNPq), Coordination for the Improvement of Higher Education Personnel (CAPES), and the Foundation for Research Support of the Federal District (FAPDF).
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All authors contributed to the study’s conception and design. Material preparation, data collection, and analysis were performed by Antonielle Vieira Monclaro, Pedro Ribeiro Fontes, and Guilherme Lima Recalde. Antonielle Vieira Monclaro wrote the first draft of the manuscript and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Monclaro, A.V., Fontes, P.R., Recalde, G.L. et al. Evaluation of endoglucanase and xylanase production by Aspergillus tamarii cultivated in agro-industrial lignocellulosic biomasses. Folia Microbiol 67, 721–732 (2022). https://doi.org/10.1007/s12223-022-00971-8
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DOI: https://doi.org/10.1007/s12223-022-00971-8