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Contribution of zinc solubilizing bacterial isolates on enhanced zinc uptake and growth promotion of maize (Zea mays L.)

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Abstract

Zinc-solubilizing bacteria, namely Burkholderia cepacia and Acinetobacter baumannii (H1 and H3, respectively), able to grow in liquid basal medium supplemented with ZnO, Zn3 (PO4)2, and ZnSO4·7H2O (0.1%), showed plant growth promoting properties. The treatment of Acinetobacter baumannii (H3) solubilizes the ZnO (1.42 ppm), Zn3 (PO4)2 (1.15 ppm), and ZnSO4·7H2O (1.44 ppm).The maximum solubilization of ZnSO4·7H2O (1.42 ppm) was observed in Burkholderia cepacia (H1) after 15 days. Organic acids produced by the bacteria decreased the pH of the medium and helped in Zn solubilization. In pot experiment on maize, Burkholderia cepacia (H1) treatment significantly enhanced plant height and root length in the presence of ZnO (2%) added in 10 mL of inoculum in each pot. High-performance liquid chromatography (HPLC) analysis of maize root extract showed the presence of oxalic, maleic, tartaric, and fumaric acid after 60 days of the experiment. Bacterial treatments enhanced sugar and protein level in maize plants and were 55.2 and 42.55 µg/mL plant extract, respectively, under mixture of bacterial treatment. Hence, isolates H1 and H3 expressed highest potential throughout the experiments, as zinc solubilizers and plant growth-promoting strains. This study demonstrated that meticulous use of Zn-solubilizing bacterial strains could aid in enhanced plant growth and can be the potential bio-inoculants for biofortification of maize to overcome the problems of malnutrition.

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Abbreviations

U :

Unsterilized soil

(+) :

Positive control

(-) :

Negative control

H1H3 :

Bacterial cultures

Z :

Zinc treatment

UC :

Neutral (no zinc and no bacteria)

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Acknowledgements

Authors are thankful for the facilities provided by the Departments of Microbiology, Soil Science and Chemistry of GBPUA &T Pantnagar to conduct the work.

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Authors and Affiliations

  1. Department of Microbiology, GBPUAT, Pantnagar, 263145, India

    Himantika Upadhyay, Anita Sharma & Damini Maithani

  2. School of Agriculture, Graphic Era Hill University, Bhimtal, 263136, India

    Saurabh Gangola

  3. ICAR-National Bureau of Agriculturally Important Microorganisms, Uttar Pradesh, Maunath Bhanjan, 275101, India

    Arjun Singh

  4. ICAR-Central Soil Salinity Research Institute, RRS Lucknow, Lucknow, India

    Arjun Singh

  5. Department of Biosciences, Shri Ram Group of Colleges, Muzaffarnagar, 241001, India

    Samiksha Joshi

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  1. Himantika Upadhyay
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  2. Saurabh Gangola
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  3. Anita Sharma
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  4. Arjun Singh
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  5. Damini Maithani
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  6. Samiksha Joshi
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Correspondence to Samiksha Joshi.

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Upadhyay, H., Gangola, S., Sharma, A. et al. Contribution of zinc solubilizing bacterial isolates on enhanced zinc uptake and growth promotion of maize (Zea mays L.). Folia Microbiol 66, 543–553 (2021). https://doi.org/10.1007/s12223-021-00863-3

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