Abstract
The aim of this work was to compare production of endotoxin and to determine susceptibility to antibiotics in two groups of specimens—wild-type strains Ochrobactrum anthropi isolated from the environment and the strains isolated from patients with cystic fibrosis. The determination of the endotoxin produced by the test strains was carried on by using a limulus amebocyte lysate test (LAL test). Determination of ATB sensitivity was accomplished by means of a broth dilution method in a microtiter plate (MIC). No significant difference was found between the group of ochrobacters isolated from the environment and the group of ochrobacters isolated from cystic fibrosis patients. Antibiotic sensitivity testing has indicated that the resistance to tigecycline, trimethoprim/sulfamethoxazole, and gentamicin was slightly higher in strains isolated from cystic fibrosis patients in comparison with strains isolated from the environment. In general, most of the test strains were sensitive to most of the antibiotics tested. Significant resistance has been demonstrated for cefotaxime. Resistance was also found for gentamicin in strains number 4 and 7. The MIC was equal to the breakpoint for this antibiotic (8000 mg/L).
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Acknowledgments
The research did not receive specific funding, but was performed as part of the employment of the authors of the Faculty of Medicine, University of Ostrava, Czech Republic; Department of Public Health, Faculty of Medicine and Dentistry, Palacký University Olomouc, Czech Republic; Centre of Hyperbaric Medicine, Ostrava City Hospital, Czech Republic; Department of Neonatology, University Hospital Olomouc, Czech Republic; Swedish Orphan Biovitrum AB, Stockholm, Sweden; and Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University Olomouc, Czech Republic.
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DCh: the author of the article and scenario of experiments and article writing; OH: the corresponding author, suggestions and checking the manuscript; IK, AL, MH: organization of experimental work in the Faculty of Medicine of University of Ostrava; CG-R, JL, VR, IČ: suggestions and checking the manuscript.
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Chmelař, D., Holý, O., Kasáková, I. et al. Antibiotic susceptibility and production of endotoxin by Ochrobactrum anthropi isolated from environment and from patients with cystic fibrosis. Folia Microbiol 64, 861–865 (2019). https://doi.org/10.1007/s12223-019-00700-8
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DOI: https://doi.org/10.1007/s12223-019-00700-8