Abstract
The effect of the administration of chitosan (CS) and chitooligosaccharides (COS) on rat fecal microbiota was analyzed in this study. The profile of total bacterial population was monitored during 3 weeks of CS or COS application using denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene amplicons. Quantitative PCR was used for monitoring possible changes in the levels of total bacteria and the levels of individual bacterial groups: Bifidobacteria, Clostridium leptum, Enterobacteriaceae, Lactobacillus–Streptococcus–Enterobacter, and Bacteroides–Prevotella. The DGGE profiles revealed a high complexity and individuality of each tested subject, and variations in the composition of band pattern were observed. CS or COS per os administration changed the profile and structure of the microbial ecosystem of the gastrointestinal tract of healthy rats. COS have, in most cases, an opposite effect compared with CS; only the Bacteroides–Prevotella bacterial group and Enterobacteriaceae were influenced in the same way. The Bifidobacteria group was not influenced by the administration CS and COS.
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Bartosch S, Fite A, Macfarlane GT, McMurdo MET (2004) Characterization of bacterial communities in feces from healthy elderly volunteers and hospitalized elderly patients by using realtime PCR and effects of antibiotic treatment on the fecal microbiota. Appl Environ Microbiol 70:3575–3581. doi:10.1128/AEM.70.6.3575-3581.2004
Duncan SH, Louis P, Flint HJ (2007) Cultivable bacterial diversiry from the human colon. Lett Appl Microbiol 44:343–350. doi:10.1111/j.1472-765X.2007.02129.x
Hattori M, Taylor TD (2009) The human intestinal microbiome? A new frontier of human biology. DNA Res 16:1–12. doi:10.1055/s-0029-1185387
Kaufmann P, Pfefferkorn A, Teuber M, Meile L (1997) Identification and quantification of Bifidobacterium species isolated from food with genus-specific 16SrRNA-targeted probes by colony hybridization and PCR. Appl Environ Microbiol 63:1268–1273
Kim SK, Rajapakse N (2005) Enzymatic production and biological activities of chitosan oligosaccharides (COS): a review. Carbohydrate Polym 62:357–368. doi:10.1016/j.carbpol.2005.08.012
Lee HW, Park YS, Jung JS, Shin WV (2002) Chitosan oligosaccharides, dp 2-8, have prebiotic effect on the Bifidobacterium bifidum and Lactobacillus sp. Anaerobe 8:319–324. doi:10.1016/S1075-9964(03)00030-1
Ley RE, Turnbaugh P, Klin S, Gordon JI (2006) Microbial ecology—human gut microbes associated with obesity. Nature 444:1022–1023. doi:10.1038/4441022a
Moore WEC, Moore LH (1995) Intestinal floras of population that have a high-risk of colon-cancer. Appl Environ Microbiol 61:3202–3207
Mrázek J, Štrosová L, Fliegerová K, Kott T, Kopečný J (2008) Diversity of insect intestinal microflora. Folia Microbiol 53:229–233. doi:10.1007/s12223-008-0032-z
Mrázek J, Koppová I, Kopečný J, Šimůnek J, Fliegerová K (2010) PCR-DGGR based study of fecal microbial stability during the long-term chitosan supplementation of humans. Folia Microbiol 55:352–358
Rhoades J, Roller S (2000) Antimicrobial action of degraded and native chitosan against spoilage organisms in laboratory media and food. Appl Environ Microbiol 66:80–86
Rinttilä T, Kassinen A, Malinen E, Krogilus L, Pavla A (2004) Development of extensive set of 16S rDNA-targeted primers for quantification of pathogenic and indigenous bacteria in faecal sapmes by real-time PCR. J Appl Microbiol 97:1166–1177. doi:10.1111/j.1365-2672.2004.02409.x
Salzman NH, Kuiechun H, Haribhai D et al (2010) Enteric defensins are essential regulators of intestinal microbial ecology. Nat Immunol 11:76–82. doi:10.1038/ni.1825
Šimůnek J, Tischenko G, Hodrová B, Bartoňová H (2006) Effect of chitosan on the growth of humn colonic bacteria. Folia Microbiol 51:306–308. doi:10.1007/BF02931820
Šimůnek J, Koppová I, Filip L, Tischenko G, Belzecki G (2010) The antimicrobial action of low-molar-mass chitosan, chitosan derivatives and chitooligosaccahrides on bifidobactera. Folia Microbiol 55:379–382
Qin C, Du Y, Xiao L, Li Z, Gao X (2002) Enzymatic preparation of water-soluble chitosan and their antitumor activity. Int J Macromol 31:111–117. doi:10.1016/S0141-8130(02)00064-8
Zoetendal EG, Aggermans ADL, De Vos DM (1998) Temperature gradient gel electrophoresis analysis of 16S rRNA from human fecal samples reveals stable and host-specific communities of active bacteria. Appl Environ Microbiol 64:3854–3859
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This work was supported by the Grant Agency of the Czech Republic (project no. 310/09/1407). It was also supported by the Grant Agency of the Czech Republic (project no. 310/09/1407).
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Koppová, I., Bureš, M. & Šimůnek, J. Intestinal bacterial population of healthy rats during the administration of chitosan and chitooligosaccharides. Folia Microbiol 57, 295–299 (2012). https://doi.org/10.1007/s12223-012-0129-2
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DOI: https://doi.org/10.1007/s12223-012-0129-2