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Participation of cell-surface hydrophobins for hemin binding in Helicobacter pylori

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Abstract

Treatment of Helicobacter pylori cells with several chaotropic agents resulted in different degrees of inhibition in the binding of the bacteria to hemin and Congo-red dye. Polyanions also yielded a >50% inhibitory effect. Furthermore, hydrophobic interaction chromatography was used to determine the relative surface hydrophobicity of cell-associated proteins extracted with 3 mol/L urea, revealing proteins with a significant hydrophobic profile.

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Abbreviations

CR:

Congo-red

HIC:

Hydrophobic interaction chromatography

HM:

Hemin

H.p. :

Helicobacter pylori

HS:

Heparan sulfate

PBS:

Phosphate-buffered saline

PEG:

Poly(ethylene)glycol

SDS-PAGE:

Sodium dodecyl sulfate polyacrylamide gel electrophoresis

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This work was supported by the Center for Biological Research, CIBNOR (ABM-11). The first author was recipient of a scholarship (59291) from the Mexican Council of Science and Technology (CONACyT). We thank Tec. A.A. Cruz for assistance on chromatographic procedures.

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Authors and Affiliations

  1. Departamento de Ciencias Químico Biológicas, Universidad de Sonora, Blvd. Luis Encinas y Rosales s/n Hermosillo, Sonora, 83000, Mexico

    E. Ruiz-Bustos

  2. Department of Medical Microbiology, University of Lund, 223 62, Lund, Sweden

    T. Wadström

  3. Laboratorio de Patogénesis Microbiana, Centro de Investigaciones Biológicas del Noroeste, La Paz, Baja California Sur, Mexico

    F. Ascencio

Authors
  1. E. Ruiz-Bustos
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  2. T. Wadström
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  3. F. Ascencio
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Correspondence to E. Ruiz-Bustos.

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Ruiz-Bustos, E., Wadström, T. & Ascencio, F. Participation of cell-surface hydrophobins for hemin binding in Helicobacter pylori . Folia Microbiol 56, 241–245 (2011). https://doi.org/10.1007/s12223-011-0043-z

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  • DOI: https://doi.org/10.1007/s12223-011-0043-z

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