Abstract
Three Lactobacillus strains (LOCK 0900, LOCK 0908, LOCK 0919) out of twenty-four isolates were selected according to their antagonistic activity against pathogenic bacteria, resistance to low pH and milieu of bile salts. Intragastric administration of a mixture of these strains to Balb/c mice affected cytokine TH1-TH2 balance toward nonallergic TH1 response. Spleen cells, isolated from lactobacilli-treated mice and re-stimulated in vitro with the mixture of heat-inactivated tested strains, produced significantly higher amounts of anti-allergic tumor necrosis factor- and interferon-γ than control animals whereas the level of pro-allergic interleukin-5 was significantly lower. Lactobacillus cells did not translocate through the intestinal barrier into blood, liver and spleen; a few Lactobacillus cells found in mesenteric lymph nodes could create antigenic reservoir activating the immune system. The mixture of Lactobacillus LOCK 0900, LOCK 0908 and LOCK 0919 strains represents a probiotic bacterial preparation with possible use in prophylaxis and/or therapy of allergic diseases.
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Abbreviations
- CFU:
-
colony forming units
- Con:
-
A concanavalin A
- IFN:
-
interferon
- IL:
-
interleukin
- MLN:
-
mesenteric lymph nodes
- PBS:
-
phosphate-buffered saline
- TH :
-
T helper (lymphocytes)
- TGF:
-
transforming growth factor
- TNF:
-
tumor necrosis factor
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Cukrowska, B., Motyl, I., Kozáková, H. et al. Probiotic Lactobacillus strains: in vitro and in vivo studies. Folia Microbiol 54, 533–537 (2009). https://doi.org/10.1007/s12223-009-0077-7
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DOI: https://doi.org/10.1007/s12223-009-0077-7