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The effects of thiamine inhibition on ruminal fermentation: a preliminary study

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Abstract

Inhibition of methanogenesis in ruminal cultures was attempted by hindering thiamine availability through its degradation by ‘polyphenols’ and competition for active sites on enzymes and transporters using thiamine structural analogs. Effects on fermentation were small and not consistently reversed by adding thiamine. Lack of major effects of the compounds evaluated could be due to intracellular synthesis of thiamine covering most requirements.

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Abbreviations

CP:

crude protein

SEM:

standard error of the mean

DM:

dry matter TPP thiamine diphosphate

HPLC:

high performance liquid chromatography

VFA:

volatile fatty acid

NDF:

neutral detergent fiber

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Authors and Affiliations

  1. Department of Animal Science, Michigan State University, East Lansing, MI, 48824, USA

    E. M. Ungerfeld, S. R. Rust & R. Burnett

  2. Lethbridge Research Centre, Lethbridge, Alberta, T1J 4B1, Canada

    E. M. Ungerfeld

Authors
  1. E. M. Ungerfeld
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  2. S. R. Rust
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  3. R. Burnett
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Correspondence to E. M. Ungerfeld.

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Ungerfeld, E.M., Rust, S.R. & Burnett, R. The effects of thiamine inhibition on ruminal fermentation: a preliminary study. Folia Microbiol 54, 521–526 (2009). https://doi.org/10.1007/s12223-009-0075-9

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  • DOI: https://doi.org/10.1007/s12223-009-0075-9

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