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Silk fibroin/hyaluronic acid porous scaffold for dermal wound healing

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Abstract

The use of silk protein as a biomaterial has been studied for decades. In this study, silk fibroin (SF)/hyaluronic acid (HA) blend scaffolds were prepared by freeze-drying technique. The structure and properties of the blend scaffolds were examined and analyzed. The results demonstrated that the secondary structures of the SF/HA scaffolds were mainly amorphous and β-sheet structures. The pore radius and porosity of the scaffolds decreased with a decrease in the freezing temperature decrease and an increase in the HA ratio. The pore radius and porosity were regulated from 32.22 μm to 290.76 μm and from 74.1 % to 91.15 %, respectively. In vitro, the SF/HA scaffolds could support the fibroblast cell adhesion and proliferation and showed good cytocompatibility. In vivo, the SF/HA scaffolds were implanted into the dorsum of Sprague Dawley rats to evaluate their bioactivity for dermal tissue reconstruction. The vascular-like structures appeared more rapidly in SF/HA scaffolds than that in the PVA group, and a new dermal layer was formed, as determined by histological analysis. The SF/HA porous scaffolds have promise as a dermal substitute.

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Authors and Affiliations

  1. National Engineering Laboratory for Advanced Yarn and Fabric Formation and Clean Production, School of Textile Science and Engineering, Wuhan Textile University, Wuhan, 430073, China

    Qiang Zhang, Shu Chen, Renchuan You, Zeeshan Tariq, Jingjing Huang & Shuqin Yan

  2. National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou, 215123, China

    Mingzhong Li

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  1. Qiang Zhang
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  2. Shu Chen
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  3. Renchuan You
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  4. Zeeshan Tariq
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  5. Jingjing Huang
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  6. Mingzhong Li
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  7. Shuqin Yan
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Correspondence to Shuqin Yan.

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Zhang, Q., Chen, S., You, R. et al. Silk fibroin/hyaluronic acid porous scaffold for dermal wound healing. Fibers Polym 18, 1056–1063 (2017). https://doi.org/10.1007/s12221-017-1230-6

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  • DOI: https://doi.org/10.1007/s12221-017-1230-6

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